Addgene

We narrowed to 1,292 results for: cs

Showing: 1021 - 1040 of 1292 results

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pFB-CG-LYCHOS FP>IA-HA-GFP

Plasmid

#199688

Purpose

Expression of LYCHOS FP>IA mutant in insect cells

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Use

Tags

10xHis, 6xHis, EGFP, HA, and HRV 3C

Expression

Insect

Mutation

F43 and P44 mutated to I and A respectively

Promoter

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a LZ LED 4CA-FLAG

Plasmid

#199681

Purpose

Bacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper LYCHOS LED 4CA (GPR155 Human)

Use

Tags

6xHis and Flag

Expression

Bacterial

Mutation

C595.604.629.638 of LYCHOS mutated to A

Promoter

T7 promoter

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a LZ LED Y551A-FLAG

Plasmid

#199680

Purpose

Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper LYCHOS LED Y551A (GPR155 Human)

Use

Tags

6xHis and Flag

Expression

Bacterial

Mutation

Y551 of LYCHOS mutated to A

Promoter

T7 promoter

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut2

Plasmid

#180425

Purpose

mammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_GFP11-14-SEPT7

Plasmid

#180342

Purpose

mammalian expression of human SEPT7 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-14-GFP11

Plasmid

#180344

Purpose

mammalian expression of human SEPT9_i1 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-14-GFP10

Plasmid

#180345

Purpose

mammalian expression of human SEPT9_i3 fused to GFP10

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

GFP10

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-14-GFP11

Plasmid

#180346

Purpose

mammalian expression of human SEPT9_i3 fused to GFP11

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut1

Plasmid

#180316

Purpose

mammalian expression of human SEPT7 Gmut1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 W269A, H279D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple

Plasmid

#180323

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple-CAAX

Plasmid

#180324

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple and CAAX (HRas)

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pnCS_DSep2_Peanut-TEV-Strep

Plasmid

#174495

Purpose

bacterial co-expression of Drosophila Sep2 and of Drosophila Peanut

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

Sep2 (Sep2 Fly)
peanut (pnut Fly)

Use

Tags

TEV-Strep

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA-NONO-1

Plasmid

#127654

Purpose

Knock-out of human NONO (guide only)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO sgRNA (NONO Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTR-UF11 (AAV.A10.T)

Viral Prep

#157970-AAV.A10.T

Purpose

Ready-to-use AAV6(dbY-F+T-V) in TMN200 particles produced from pTR-UF11 (#157970). In addition to the viral particles, you will also receive purified pTR-UF11 plasmid DNA. 'Humanized' GFP driven by Chimeric CMV/Chicken Beta Actin promoter

Depositor

Sergei Zolotukhin

Article

Burger et al Mol Ther. 2004 Aug;10(2):302-17. doi: 10

Promoter

chimeric CMV/Chicken Beta actin (CBA)

Tags

GFP

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only
pTR-UF11 (AAV.A08.T)

Viral Prep

#157970-AAV.A08.T

Purpose

Ready-to-use AAV2(4pMut)dHS in BSS particles produced from pTR-UF11 (#157970). In addition to the viral particles, you will also receive purified pTR-UF11 plasmid DNA. 'Humanized' GFP driven by Chimeric CMV/Chicken Beta Actin promoter

Depositor

Sergei Zolotukhin

Article

Burger et al Mol Ther. 2004 Aug;10(2):302-17. doi: 10

Promoter

chimeric CMV/Chicken Beta actin (CBA)

Tags

GFP

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only
iGFP-beta-actin

Plasmid

#231553

Purpose

Mammalian expression of human beta actin fused intramolecularly to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

ACTB (ACTB Human)

Use

Tags

msfGFP (intramolecular)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1.sh.beta-catenin.1248

Plasmid

#19761

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against beta-catenin (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pLKO.1.sh.beta-catenin.2279

Plasmid

#19762

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against beta-catenin (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
MSCV-BCL6-t2A-BCL2

Plasmid

#135305

Purpose

Overexpression of multiple human genes (BCL2 and BCL6)

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

BCL6 (BCL6 Human)
BCL2 (BCL2 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

LTR

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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123_pAAV-ProC1-CatCh-GFP-WPRE

Plasmid

#125937

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC1

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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