We narrowed to 15,752 results for: genome

1. pSEVA6213S

   Plasmid

   #122094

   Purpose

   constitutive expression of I-SceI for genome engineering in Pseudomonas

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Insert

   I-SceI restriction endonuclease
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Pem7

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. NRASA

   Plasmid

   #25256

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NRASA:M1-N172 (NRAS Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids M1-N172

   Promoter

   Available Since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pSEVA451-Cpf1

   Plasmid

   #192362

   Purpose

   CRISPR-based vector for genome editing

   Depositor

   Juana María Navarro Llorens

   Article

   Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

   Insert

   Cas12a, gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGNW2

   Plasmid

   #122086

   Purpose

   suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVA351-Cpf1

   Plasmid

   #192361

   Purpose

   CRISPR-based vector for genome editing

   Depositor

   Juana María Navarro Llorens

   Article

   Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

   Insert

   Cas12a, gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTKLP-tetA

   Plasmid

   #71325

   Purpose

   tetA landing pad template plasmid

   Depositor

   Thomas Kuhlman

   Article

   Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTKDP-neo

   Plasmid

   #71320

   Purpose

   Donor plasmid - kanamycin resistant donor cassette

   Depositor

   Thomas Kuhlman

   Article

   Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTKDP-dhfr

   Plasmid

   #71323

   Purpose

   Donor plasmid - trimethoprim resistant donor cassette

   Depositor

   Thomas Kuhlman

   Article

   Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGNW4

   Plasmid

   #122088

   Purpose

   suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DDB1

    Plasmid

    #25246

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DDB1_1 (DDB1 Human)
    

    Use

    Baculovirus expression

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAH69

    Plasmid

    #173782

    Purpose

    The pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    HK022 Integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBTK1050

    Plasmid

    #209540

    Purpose

    BTK Type 1 homology arm for Golden Gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al bioRxiv 2023.09.19.558440

    Insert

    Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pCHAP3_Sap

    Plasmid

    #206849

    Purpose

    A plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCHAP2

    Plasmid

    #206847

    Purpose

    A plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCHAP1

    Plasmid

    #206846

    Purpose

    A plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmCherry-GFP

    Plasmid

    #86441

    Purpose

    visualization and quantifying the out-of-frame mutations from genome editing

    Depositor

    Yun-Bo Shi

    Article

    Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

    Insert

    mCherry-MCS-sfGFP fusion gene
    

    Use

    Color visualization in bacteria

    Tags

    mCherry-sfGFP

    Expression

    Mutation

    Promoter

    Plac

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSEVA1213S

    Plasmid

    #122095

    Purpose

    constitutive expression of I-SceI for genome engineering in Pseudomonas

    Depositor

    Pablo Ivan Nikel

    Article

    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    I-SceI restriction endonuclease
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGNW6

    Plasmid

    #122093

    Purpose

    suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

    Depositor

    Pablo Ivan Nikel

    Article

    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EHMT1 (2IGQ)

    Plasmid

    #25504

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EHMT1 (EHMT1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C-terminal domain, residues 951-1235

    Promoter

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pLacZa-GFP

    Plasmid

    #86440

    Purpose

    visualization and quantifying the out-of-frame mutations from genome editing

    Depositor

    Yun-Bo Shi

    Article

    Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

    Insert

    LacZalpha-MCS-sfGFP fusion gene
    

    Use

    Color visualization in bacteria

    Tags

    LacZa-sfGFP

    Expression

    Mutation

    Promoter

    Plac

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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