We narrowed to 1,029 results for: gibson assembly

1. NDV-HN-SpyTag

   Plasmid

   #160000

   Purpose

   Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   NDV-HN-SpyTag
   

   Use

   Tags

   His6-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta-actin promoter

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyTag003-OspC

   Plasmid

   #159998

   Purpose

   Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyTag003-OspC (20-209) C129S
   

   Use

   Tags

   His6-tag and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   C129S mutation improves expression level.

   Promoter

   T7

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P1 UBXN11

   Plasmid

   #169017

   Purpose

   Expresses GST-UBXN11 (human) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

   Insert

   UBXN11 (UBXN11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIN-TRE-EB3N-VVDfast-mCl3-iLID

   Plasmid

   #190169

   Purpose

   Doxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   EB3N-VVDfast-mClover3-iLID
   

   Use

   Lentiviral

   Tags

   -VVDfast-mClover3-iLID

   Expression

   Mutation

   1-189 aa from EB3

   Promoter

   TRE

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

   Plasmid

   #204288

   Purpose

   yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

   Depositor

   Linda Huang

   Article

   Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

   Insert

   pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   pHis3

   Available since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpySwitch

   Plasmid

   #184225

   Purpose

   Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   SpySwitch
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E77A mutation prevents isopeptide bond formation,…

   Promoter

   T7

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpyTag-RBD

   Plasmid

   #159999

   Purpose

   Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFL His-mEos3.2-I3

   Plasmid

   #113516

   Purpose

   Expression of His-Eos-I3 in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   I3 (PPP1R11 Human)
   

   Use

   Tags

   6*His-mEos3.2

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMMK ENTR 1 CMV mAG h-Geminin

   Plasmid

   #206281

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly. Encodes mAG-hGeminin under the control of CMV

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   mAG-hGeminin (GMNN Human)
   

   Use

   Multimate/gateway entr 1

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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