We narrowed to 1,086 results for: gibson assembly

1. pGL3-NLS-ccdb-DddA(G1333N)-UGI-Puro

   Plasmid

   #173233

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-NLS-ccdb-DddA(G1397C)-UGI-Puro

   Plasmid

   #173234

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL3-NLS-ccdb-DddA(G1397N)-UGI-Puro

   Plasmid

   #173235

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEND-320_Cacnes_ori

   Plasmid

   #225602

   Purpose

   Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli ori

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEND-321_Cacnes_ori

   Plasmid

   #225603

   Purpose

   Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEND-350_Cacnes_ori

   Plasmid

   #225605

   Purpose

   Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUb-Cas9-EGFP_cU6:6-sgRNA

   Plasmid

   #190597

   Purpose

   The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   EGFP - separated by T2A

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pG2J

   Plasmid

   #102882

   Purpose

   Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Inserts

   OsTIR1
   mGFP-AID
   

   Tags

   AID(71-114)

   Expression

   Yeast

   Promoter

   ADH1 and TEF1

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUb-Cas9-mCherry_cU6:6-sgRNA

   Plasmid

   #190598

   Purpose

   The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   mCherry - separated by T2A

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM4941 (pCV0053)

    Plasmid

    #120083

    Purpose

    Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI
    

    Use

    Other

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM4937 (pCV0049)

    Plasmid

    #120082

    Purpose

    Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI
    

    Use

    Other

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAM4951 (pCV0063)

    Plasmid

    #120084

    Purpose

    Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.
    

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPOLY_new

    Plasmid

    #179276

    Purpose

    Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    cellodextrin phosphorylase
    cellobiose phosphorylase
    sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
    

    Use

    Synthetic Biology

    Tags

    6xHIS and Strep-Tag II

    Expression

    Bacterial

    Promoter

    T7 lacO

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM4891 (pCV0003)

    Plasmid

    #120080

    Purpose

    Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2
    

    Use

    Other

    Mutation

    mobA Y25F

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

    Plasmid

    #231364

    Purpose

    Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    mRuby2
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNA0304

    Plasmid

    #165612

    Purpose

    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    NotI site replaced the CAN1 spacer in parent vect…

    Promoter

    SNR52

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAM4896 (pCV0008)

    Plasmid

    #120081

    Purpose

    Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a nourseothricin resistance gene in various cyanobacteria.

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-nat1_A7120, CV-PconII-LTRBS-GFPmut2
    

    Use

    Other

    Mutation

    mobA Y25F

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAM4889 (pCV0001)

    Plasmid

    #120079

    Purpose

    Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a spectinomycin/streptomycin resistance gene in various cyanobacteria.

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aadA, CV-PconII-LTRBS-GFPmut2
    

    Use

    Other

    Mutation

    mobA Y25F

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGTPA-eGFP

    Plasmid

    #171526

    Purpose

    Plasmid contains the Cas12k gRNA and transposon donor cassette assembled with eGFP

    Depositor

    Quanjiang Ji

    Article

    Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

    Insert

    Cas12k gRNA, transposon donor cassette, eGFP
    

    Use

    CRISPR

    Promoter

    Constitutive promoter J23119

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGTPA-Gm

    Plasmid

    #171527

    Purpose

    Plasmid contains the Cas12k gRNA and transposon donor cassette assembled with gentamycin-resistant gene

    Depositor

    Quanjiang Ji

    Article

    Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

    Insert

    Cas12k gRNA, transposon donor cassette, gentamycin-resistant gene
    

    Use

    CRISPR

    Promoter

    Constitutive promoter J23119

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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