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PB-TAC-WTSI

Plasmid

#80486

Purpose

piggyBac transposon for dox-inducible expression of the WTSI polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

WTSI

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.9

Plasmid

#78179

Purpose

Expresses-rsGreen0.9 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.9

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.7b

Plasmid

#78178

Purpose

Expresses rsGreen0.7b in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.7b

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pSCKtheoRaj12-olsa

Plasmid

#69940

Purpose

pSTC2,theoHHAzRaj12,cisRaj12-sfGFP, KanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Inserts

TheoHHAzRaj12,cisRaj12,sfGFP
theoHHAzRaj12,cisRaj12-sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pGEM-and3 in situ probe

Plasmid

#52104

Purpose

actinodin 3 and3 antisense in situ probe linearize w/ ApaI SP6 pol

Depositor

Didier Stainier

Article

Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

Insert

actinodin 3 in situ probe (and3 Zebrafish)

Expression

Bacterial

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pPK1E-2

Plasmid

#48146

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pPK1E-1

Plasmid

#48145

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt4 Non-STOP

Plasmid

#35892

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Use

Gateway Cloning

Mutation

Silent Mutation C (492) --> T

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt8A Non-STOP

Plasmid

#35898

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8A (Wnt8a Human)

Use

Gateway Cloning

Mutation

Silent Mutation A (600) --> G

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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sankE3_5

Plasmid

#24893

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

sank E3_5

Tags

His

Expression

Bacterial

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
PrbC5

Plasmid

#24787

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Tags

His

Expression

Bacterial

Mutation

Asp11Gly, Asp83Asn, Val92Ala, Glu119Gly, and Glu1…

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pENTR4_EWSR1_C2G_R650G

Plasmid

#26371

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Gateway Cloning

Mutation

R650G

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pET-17b_K490clm_E215C

Plasmid

#169995

Purpose

Kinesin KIF5B K490 cysteine light mutant with E215C mutation for single cysteine labeling

Depositor

Ron Vale

Article

Niekamp et al Proc Natl Acad Sci U S A. 2019 Mar 5;116

Insert

kinesin family member 5B, isoform CRA_a

Tags

6xHIS

Expression

Bacterial

Mutation

C7S, C65A, C168A, C174S, E215C, C294A, C330S, C42…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS1

Plasmid

#145362

Purpose

Cell-free/mammalian expression vector for Zika virus NS1 protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS1

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Availability

Academic Institutions and Nonprofits only

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pmCell-Free_KA2_ZIKV NS2B

Plasmid

#145364

Purpose

Cell-free/mammalian expression vector for Zika virus NS2B protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2B

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS3

Plasmid

#145365

Purpose

Cell-free/mammalian expression vector for Zika virus NS3 protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS3

Tags

8xHis and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Availability

Academic Institutions and Nonprofits only

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PB-TO-hNGN2

Plasmid

#172115

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

hNGN2 (NEUROG2 Human)
puroR-T2A-mycNLS-mTagBFP2

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PG13-mCherry-EF1a-PuroR-EGFP

Plasmid

#195292

Purpose

piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

mCherry
PuroR-P2A-EGFP

Use

Piggybac transposon

Expression

Mammalian

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSIR-bsr

Plasmid

#176507

Purpose

Self-inactivating retrovirus vector containing a Blasticidin S-resistance gene

Depositor

Hodaka Fujii

Article

Fujii et al Biol Methods Protoc. 2021 Dec 6;6(1):bpa

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCoofy22

Plasmid

#43992

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10 and S

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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