We narrowed to 15,951 results for: grna

1. RIB VP64 R1

   Plasmid

   #161493

   Purpose

   CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2

   Depositor

   Diethard Mattanovich, Brigitte Gasser

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Targeting R1 in the RIB1 promoter)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGAP, pPOR1, and pTEF2

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ135A

   Plasmid

   #69277

   Purpose

   Golden Gate entry vector; 5th gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ133C

   Plasmid

   #69286

   Purpose

   Golden Gate entry vector; 1st gRNA under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ136A

   Plasmid

   #69278

   Purpose

   Golden Gate entry vector; 6th gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEG302 22aa SunTag VP64 g4+g17

   Plasmid

   #120252

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRetroSuper-GFP Smad3

   Plasmid

   #15723

   Depositor

   Joan Massague

   Article

   He et al Cell. 2006 Jun 2. 125(5):929-41.

   Insert

   Smad3 shRNA (SMAD3 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pALPS puro miR30-L1221

   Plasmid

   #101335

   Purpose

   negative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGT

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   shRNA targeting miR30
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSuper-gmiR-124

   Plasmid

   #171555

   Purpose

   a human miR-124-expressing plasmid

   Depositor

   Yan Zeng

   Article

   Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

   Insert

   miR-124 (MIR124-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiX2-PURO-shGFP

    Plasmid

    #61230

    Purpose

    Lentivirus expressing shRNA to GFP

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pADH137

    Plasmid

    #90986

    Purpose

    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    C. albicans LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. px459 VRER

    Plasmid

    #101716

    Purpose

    sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E and T1337R)

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-PICK

    Plasmid

    #31613

    Depositor

    Robert Malenka

    Article

    Citri et al J Neurosci. 2010 Dec 8. 30(49):16437-52.

    Insert

    PICK1 (shRNA insensitive) +shRNA towards PICK1 (Pick1 Rat)
    

    Use

    Lentiviral

    Tags

    GFP, HA, and PICK1 shRNA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCCM935

    Plasmid

    #58202

    Purpose

    Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    unc-22 sgRNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pADH140

    Plasmid

    #90988

    Purpose

    NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    C. maltosa LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. dCas9v2

    Plasmid

    #103140

    Purpose

    TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    dCas9-VPR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Removed scaffold gRNA from original plasmid

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330A_D10A-1x7

    Plasmid

    #58777

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA6.2-GW/EmGFP-miR-SUMO23

    Plasmid

    #31073

    Depositor

    Wulf Paschen

    Article

    Yang et al Biochem Biophys Res Commun. 2009 Apr 24.

    Insert

    SUMO2/3 microRNA (SUMO2, SUMO3 Human)
    

    Use

    RNAi

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSUPER.PKD3.RNAi

    Plasmid

    #10818

    Depositor

    Alex Toker

    Article

    Hausser et al Nat Cell Biol. 2005 Sep . 7(9):880-6.

    Insert

    PKD3 RNAi (PRKD3 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pMM178

    Plasmid

    #61270

    Purpose

    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.

    Depositor

    Timothy Lu

    Article

    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

    Insert

    Cas9, tracrRNA, crRNA
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO, native, native (respectively)

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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