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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,472 results for: sequence

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Showing: 10541 - 10560 of 13472 results

  5. AICSDP-24:MYH10-mEGFP

    Plasmid
    #87428
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    MYH10 Homology Arms with mEGFP-linker (MYH10 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    May 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87428-plasmid-map-sequence-id-177429

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  6. AICSDP-1:PXN-EGFP

    Plasmid
    #87420
    Purpose
    Homology arms and linker-EGFP sequence for C-terminus tagging of human PXN
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    PXN Homology Arms with linker-EGFP (PXN Human)
    Use
    CRISPR; Donor template
    Tags
    linker-EGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87420-plasmid-map-sequence-id-173974

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  12. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid
    #118586
    Purpose
    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.
    Depositor
    Radhakrishnan Padmanabhan
    Article
    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.
    Insert
    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    Use
    Yeast-e. col shuttle vector prs424
    Mutation
    The conserved GDD in the NS5 polymerase gene muta…
    Available Since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    118586-plasmid-map-sequence-id-227283

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  14. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid
    #99690
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available Since
    Sept. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    99690-plasmid-map-sequence-id-194510

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  16. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

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  19. pMinDis.X513-iNicSnFR3a-(CC93)-PM

    Plasmid
    #125122
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine
    Depositor
    Henry Lester
    Article
    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018
    Insert
    iNicSnFR3a-(CC93)-PM
    Tags
    Ig-K-Chain and membrane anchoring sequence-PDGFR
    Expression
    Mammalian
    Mutation
    NA
    Promoter
    T7
    Available Since
    Oct. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    125122-plasmid-map-sequence-id-245433

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Showing: 10541 - 10560 of 13472 results