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FUW-TetO-Nanog

Plasmid

#40800

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Nanog (Nanog Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
pDAC435

Plasmid

#195238

Purpose

Mammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP

Expression

Mammalian

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D NAPA-A SV40

Plasmid

#92281

Purpose

FRET probe; extracellular membrane tethered eGFP

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Astrocyte

Use

AAV

Promoter

GfaABC1D

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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Oct4-ires-EGFP(lox neo)

Plasmid

#21547

Depositor

Rudolf Jaenisch

Article

Lengner et al Cell Stem Cell. 2007 Oct 11;1(4):403-15.

Insert

EGFP, Oct4

Use

Mouse Targeting

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Expression

Worm

Promoter

rab-3

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCHA-VRC01-scFv

Plasmid

#44874

Depositor

Dane Wittrup

Article

Mata-Fink et al J Mol Biol. 2013 Jan 23;425(2):444-56. d

Insert

VRC01 scFv

Tags

HA and Myc

Expression

Yeast

Promoter

GAL1

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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RSRF-Luc-2wt

Plasmid

#31818

Depositor

Astar Winoto

Article

Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

Insert

MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)

Use

Luciferase

Expression

Mammalian

Promoter

MEF2-dependent (-307 to -242 of Nur77/NR4A1)

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pComb3XLambda

Plasmid

#63892

Purpose

Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

Depositor

Carlos Barbas

Article

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

human antibody lambda light chain (IGLL5 Human)

Use

Phage display

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-KM

Plasmid

#20332

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Klf4-F2A-cMyc

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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Ef1a_NANOG_Ires_Puro

Plasmid

#18920

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

NANOG (NANOG Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-GFAP104-melanopsin-mCherry

Plasmid

#122630

Purpose

AAV-mediated expression of melanopsin-mCherry under the GFAP promoter

Depositor

Edward Boyden

Article

Mederos et al Glia. 2019 May;67(5):915-934. doi: 10.10

Insert

melanopsin-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

GFAP104

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag VP64 nog

Plasmid

#120251

Purpose

CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ910

Plasmid

#44481

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, NeoR, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Nanog

Plasmid

#40800

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Nanog (Nanog Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
pRG5271Neo

Plasmid

#26387

Depositor

Denis Dupuy

Article

Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

Type

Empty backbone

Expression

Bacterial and Worm

Available Since

Oct. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pAA173

Plasmid

#21743

Depositor

David Sherwood

Article

Ziel et al Nat Cell Biol. 2009 Feb . 11(2):183-9.

Insert

pie-1>mCherry::PH domain (PLC-delta)

Use

Bacterial propagation

Tags

mCherry

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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SynTagMA_post

Plasmid

#119736

Purpose

Synaptic Tag for Mapping Activity

Depositor

Thomas Oertner

Article

Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

Insert

SynTagMA

Use

AAV

Tags

FLAG

Expression

Mammalian

Promoter

hSyn-1

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHBV-Luc

Plasmid

#71414

Purpose

To see the activity of HBV transcription using Luciferase assay

Depositor

Wang-Shick Ryu

Article

Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d

Insert

HBV core promoter, enhancer I, enhancer II and luciferase

Use

Luciferase

Expression

Mammalian

Promoter

HBV

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-tdTomato-WPRE-pGHpA

Plasmid

#67527

Purpose

"Expresses the fluorescent protein tdTomato"

Depositor

Botond Roska

Article

Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

Insert

tandem dimer tomato

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDAC435

Plasmid

#195238

Purpose

Mammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP

Expression

Mammalian

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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