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DCT.VV.Orf152

Plasmid

#12840

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf152

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
E. coli Beta sliding clamp (dnaN) in pet28PP

Plasmid

#217412

Purpose

Expresses E. coli Beta sliding clamp with a His-tag in bacterial cells

Depositor

Brian Kelch

Article

Landeck et al J Biol Chem. 2024 Apr;300(4):107166. doi

Insert

Beta sliding clamp (dnaN E. coli)

Tags

His

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-nCas9-PolI5M

Plasmid

#249066

Purpose

Expresses nucleus-localized nCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

nCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI5M

Plasmid

#249060

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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p-mCherry-dCas9-PolI5M

Plasmid

#249065

Purpose

Expresses nucleus-localized dCas9 fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

dCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p-mCherry-enCas9(D10A)

Plasmid

#249063

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Neo1 gRNA

Plasmid

#246575

Purpose

CRISPR vector co-expressing Cas9 and a mouse Neo1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Neo1 gRNA (Neo1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Nrp1 gRNA

Plasmid

#246578

Purpose

CRISPR vector co-expressing Cas9 and a mouse Nrp1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Nrp1 gRNA (Nrp1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Plxna1 gRNA

Plasmid

#246574

Purpose

CRISPR vector co-expressing Cas9 and a mouse Plxna1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Plxna1 gRNA (Plxna1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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huADF S3A

Plasmid

#245943

Purpose

Expressing a constitutively active human ADF

Depositor

James Bamburg

Article

Agnew et al J Biol Chem. 1995 Jul 21;270(29):17582-7

Insert

actin depolymerizing factor (ADF) (DSTN Human)

Expression

Mammalian

Mutation

S3A

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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TtEnd_E448A_pEHISTEV

Plasmid

#179733

Purpose

TtEnd_E448A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which Glu448 has been mutated to Ala448.

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

Glu448 has been mutated to Ala448.

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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TtEnd_E329A_pEHISTEV

Plasmid

#179734

Purpose

TtEnd_E329A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which Glu329 has been mutated with Ala329

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

Glu329 has been mutated to Ala329.

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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TtEnd_H280A_pEHISTEV

Plasmid

#179735

Purpose

TtEnd_H280A_pEHISTEV is a mutant form of TtEnd5A_pEHISTEV vector. It expresess mutated TtEnd5A in which His280 has been mutated with Ala280.

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

His280 has been mutated to Ala280.

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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TtEnd_CDC_E329A

Plasmid

#179737

Purpose

TtEnd_CDC_E329A expresses TtEnd_CDC_pEHISTEV, in which Glu329 has been mutated with Ala329.

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

Glu329 has been mutated with Ala329

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pWPI_GW_BioID2_T2A_Blast

Plasmid

#214831

Purpose

Destination vector for cloning BioID2 fusion proteins

Depositor

Hani Goodarzi

Article

Khoroshkin et al Nat Commun. 2024 Sep 9;15(1):7872. doi:

Type

Empty backbone

Use

Lentiviral

Tags

BioID2

Expression

Bacterial and Mammalian

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3583)

Plasmid

#239260

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3584)

Plasmid

#239261

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-GalK-USP-ET64

Plasmid

#234662

Purpose

Expression an ELP-tagged fusion enzyme of GalK and AtUSP

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

galactokinase
UDP-sugar pyrophosphorylase (USP Mustard Weed)

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb827

Plasmid

#217500

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb837

Plasmid

#217501

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori

Depositor

Nico Claassens

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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