We narrowed to 13,988 results for: GAN

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pPBbsr2-NRG1-ScNeo

Plasmid

#209911

Purpose

To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

NRG1-ScNeo (NRG1 Human)

Tags

mNeonGreen and mScarlet

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-HBEGF-mNeonGreen

Plasmid

#209912

Purpose

To monitor the expression of HB-EGF, the plasmid encodes a recombinant HB-EGF fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

HBEGF-mNeonGreen (HBEGF Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-EREG-mNeonGreen

Plasmid

#209914

Purpose

To monitor the expression of Epiregulin, the plasmid encodes a recombinant Epiregulin fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

EREG-mNeonGreen (EREG Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.CBX8ΔChromo

Plasmid

#224708

Purpose

Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 chromodomain truncation (CBX8 Human)

Tags

HMBP

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.mEGFP-CBX8

Plasmid

#224710

Purpose

Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Tags

mEGFP

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFB1.HMBP.PrS.CBX8-KR21A

Plasmid

#224709

Purpose

Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 KR21A mt (CBX8 Human)

Tags

HMBP

Expression

Insect

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.CBX8

Plasmid

#224707

Purpose

Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Tags

HMBP

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-hsRUNX

Plasmid

#205639

Purpose

reRNA guide targeting Homo sapiens RUNX

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-hsRUNX (RUNX1 Human)

Expression

Mammalian

Promoter

U6

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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PD2529 human beta1 ecto

Plasmid

#221398

Purpose

Mammalian expression of human integrin beta1 ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 ectodomain (ITGB1 Human)

Tags

AVI tag, HRV3C cleavage site, basic coil, HA tag,…

Expression

Mammalian

Mutation

codon optimized for human mature _1 residues Q1 t…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* K418* full-length

Plasmid

#221406

Purpose

Mammalian expression of human integrin beta1 Q280* K418* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* N562* full-length

Plasmid

#221407

Purpose

Mammalian expression of human integrin beta1 N391* N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_003.gEZH2

Plasmid

#220318

Purpose

Expresses guide RNA for CRISPR/Cas9 knockout of EZH2 for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 gRNA (EZH2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EZH2-mt2

Plasmid

#220240

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2 (EZH2 Human)

Tags

6x His, MBP

Expression

Insect

Mutation

F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

Promoter

Polyhedrin

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EZH2-mt1

Plasmid

#220239

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 1 (EZH2 Human)

Tags

6x His, MBP

Expression

Insect

Mutation

R27A, R31A, F165A

Promoter

Polyhedrin

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#3

Plasmid

#208389

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#3 gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgZBP1 (Zbp1 Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EFNB2-RBD-Fc (D62Q)

Plasmid

#200979

Purpose

Mammalian expression plasmid for soluble EFNB2 (D62Q specificity mutant) fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

IgG1 Fc

Expression

Mammalian

Mutation

Soluble Receptor-Binding Domain; D62Q mutation fo…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EFNB2 (D62Q)

Plasmid

#200976

Purpose

Mammalian expression plasmid for myc-tagged EFNB2 (D62Q specificity mutant)

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

D62Q (increases specificity towards henipaviral G…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-bio-myc-mCE 211-597

Plasmid

#112716

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 211-597 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

deleted amino acids 2-210 (triphosphatase domain)

Promoter

CMV

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

Plasmid

#204214

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of DNA binding domain (delDBD)

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

Plasmid

#204191

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of R162

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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