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FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

Plasmid

#216170

Purpose

Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- FOXA.bs- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

Plasmid

#216172

Purpose

Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- Control- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

Plasmid

#216173

Purpose

Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- FOXA.bs- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-LAP2beta

Plasmid

#222416

Purpose

To express LAP2beta with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LAP2beta (Tmpo Rat)

Tags

FKBP-GFP

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-BAF

Plasmid

#222414

Purpose

To express BAF with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

BAF (BANF1 Human)

Tags

FKBP-GFP

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-UDP1.0-WPRE

Plasmid

#220849

Purpose

AAV virus packaging plasmid for neuronal expression of an extracellular UDP sensor (UDP1.0)

Depositor

Yulong Li

Article

Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

Insert

UDP1.0

Use

AAV

Promoter

human Synapsin (hSyn)

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGex-6p2_ATRX_K1033R

Plasmid

#221378

Purpose

Expresses ATRX_K1033R in bacterial cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Transcriptional regulator ATRX (ATRX Human)

Expression

Bacterial

Mutation

Changed lysine 1033 to arginine

Promoter

tac

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGex-6p2_FANCM_K819R

Plasmid

#221380

Purpose

Expresses FANCM_K819R in bacterial cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Fanconi anemia group M protein (FANCM Human)

Expression

Bacterial

Mutation

Changed lysine 819 to arginine

Promoter

tac

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEYFP_C1_ATRX

Plasmid

#221383

Purpose

Expresses ATRX in mammalian cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Transcriptional regulator ATRX (ATRX Human)

Expression

Mammalian

Mutation

K536N (Please see depositor comments)

Promoter

CMV

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-HFL1-W371A I375A D384A Y387A-GFP

Plasmid

#207025

Purpose

Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

HFL1

Tags

GFP

Expression

Yeast

Mutation

W371A I375A D384A Y387A

Promoter

pTEF1

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-GFP-Pho8-406

Plasmid

#207006

Purpose

Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Tags

GFP

Expression

Yeast

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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AA335

Plasmid

#216069

Purpose

Fragmid fragment: (2A Selection) Stop; IRES2; Start; HygroR; Stop

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop; IRES2_v1; Start; HygroR_v1.1; Stop

Use

Fragment

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX5

Plasmid

#166163

Purpose

Gateway entry vector for N. furzeriDDX5

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-DDX5(kf)-SNAP

Plasmid

#166152

Purpose

Expression of N. furzeri DDX5 in E. coli

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5

Tags

His10 and SNAP

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-EGFP

Plasmid

#166137

Purpose

EGFP expression under the control of the GPD promoter yeast

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

EGFP

Expression

Yeast

Promoter

GDP

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-mRuby3

Plasmid

#166138

Purpose

mRuby3 expression under control of the GPD promoter in yeast

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

mRuby3

Expression

Yeast

Promoter

GDP

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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p204-U6-Switch-ON

Plasmid

#217883

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Expression

Mammalian

Promoter

hU6

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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AA020

Plasmid

#216004

Purpose

Fragmid fragment: (Cas protein) binds PP7 stem loops

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

PP7 Core Protein (PCP)_v1.1

Use

CRISPR; Fragment

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AA366

Plasmid

#215970

Purpose

Fragmid fragment: (Pol 2 promoter) Gal4-induced expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

5xUAS_v1; 5xUAS_v1; 5xUAS_v1; 5xUAS_v1; Hsp70_v2; Mhc_v1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA436

Plasmid

#216035

Purpose

Fragmid fragment: (Cas protein) binds MS2 stem loops

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

MS2_v1.1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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