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pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)

Plasmid

#133797

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized AcrIIA1(T16A)

Tags

NLS(SV40)

Expression

Mammalian

Mutation

T16A

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)

Plasmid

#133799

Purpose

CMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized Leu-AcrIIA1

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)

Plasmid

#133800

Purpose

CMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized Eriv-AcrIIA1

Tags

NLS(SV40)

Expression

Mammalian

Mutation

n/a

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET28P_dCrk

Plasmid

#131145

Purpose

Expresses 6XHis::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

6XHis

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS2_K58R_Keima

Plasmid

#127141

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS2_Keima (RPS2 Human)

Use

Lentiviral

Mutation

K58R

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS2_K275R

Plasmid

#127131

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Mutation

K275R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K227R_Keima

Plasmid

#127144

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Mutation

K227R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac-LacI-LAP-HEC1

Plasmid

#114033

Purpose

Production of baculovirus with LacI-LAP-HEC1

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP-HEC1 (NDC80 Human)

Expression

Insect

Mutation

none

Promoter

CMV

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-biotin-myc- mCE

Plasmid

#82475

Purpose

Expresses mRNA capping enzyme containing N terminal biotin and myc tag

Depositor

Daniel Schoenberg

Article

Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

Insert

Biotin (Rngtt Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1alpha-HA-TFAP4(E135A/S139A)

Plasmid

#110159

Purpose

Lentiviral vector for expression of human TFAP4 (E135A/S139A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

E135A/S139A

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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