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pMX-IY HA-GBRPL2

Plasmid

#89298

Purpose

For expression of the mammalian Atg8 protein GABARAPL2 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Retroviral

Tags

HA and IRES-YFP

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-1α

Plasmid

#214341

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL1A (IL1A Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pWL078 MCP-2xGFP

Plasmid

#197047

Purpose

pCYC-NLS-MCP-2xGFP on pRS305

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

MCP-2xGFP

Expression

Yeast

Promoter

CYC1

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK2-S

Plasmid

#172426

Purpose

Expression of full-length, human GIRK2 with C-terminal StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK2 (KCNJ6 Human)

Tags

Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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AF1521-c002

Plasmid

#173109

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))

Tags

mCerulean

Expression

Bacterial

Mutation

KH-M1-L192

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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ARH3-c002

Plasmid

#173095

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRS (ADPRS Human)

Tags

mCerulean

Expression

Bacterial

Mutation

A14-S363

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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MDO1-c014

Plasmid

#173103

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD1 (MACROD1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

S58-A325, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Parp14-c012

Plasmid

#173099

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP14 (PARP14 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

W1003-A1190

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Gi-c001

Plasmid

#173078

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GNAI1 (GNAI1 Human)

Tags

mCitrine

Expression

Bacterial

Mutation

codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Parp14-c011

Plasmid

#173098

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP14 (PARP14 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

K791-F978

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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PARP9-c020

Plasmid

#173097

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP9 (PARP9 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

N310-N493

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX6 (pcDNA3.1+)

Plasmid

#75215

Purpose

Mammalian expression of SLC8B1

Depositor

Jonathan Lytton

Article

Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

Insert

SLC8B1 (Slc8b1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAWH-Rluc-let-7-A114-N40-HhR

Plasmid

#50555

Purpose

Expression of luciferase reporter mRNA in S2 cell

Depositor

Yukihide Tomari

Article

Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

Insert

Luciferase-let7-A114-N40-HhR

Use

Luciferase

Expression

Insect

Promoter

Actin5C

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pXP722

Plasmid

#46056

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

GAL1

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXP831

Plasmid

#46065

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

CUP1

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFPC3-C2cd3

Plasmid

#128871

Purpose

For mammalian expression of mouse C2cd3

Depositor

Aimin Liu

Article

Hoover et al Development. 2008 Dec;135(24):4049-58. d

Insert

C2cd3 (C2cd3 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1-mTurquoise2

Plasmid

#103974

Purpose

Plasmid encoding mTurquoise2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mTurquoise2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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9x HD-TgDeltaMOR23-Cherry

Plasmid

#15616

Depositor

Peter Mombaerts

Article

Vassalli et al Mol Cell Neurosci. 2011 Feb;46(2):381-96

Insert

9x HD-TgΔMOR23-IRES-taumCherry

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-enCas12a-A3A-Y130F

Plasmid

#193652

Purpose

Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLb-enCas12a-hA3A-Y130F, EGFP

Expression

Mammalian

Mutation

dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A-N57G

Plasmid

#193639

Purpose

Expression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-N57G, EGFP

Expression

Mammalian

Mutation

hAPOBEC3A-N57G

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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