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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,816 results for: golden gate

Showing: 1061 - 1080 of 3816 results

  1. PACE_AP_Neg_pBADProm

    Plasmid
    #242141
    Purpose
    pBAD-SD8-GIII Neg; AP Neg Circuit for AraC phage-assisted evolution
    Depositor
    Dominik Niopek
    Insert
    Gene III Negative
    Expression
    Bacterial
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242141-plasmid-map-sequence-id-486018

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  2. PACE_AP_Pos_pBADProm

    Plasmid
    #242139
    Purpose
    pBAD-sd8-GIII Cassette; AP Pos Circuit for AraC phage-assisted evolution
    Depositor
    Dominik Niopek
    Insert
    Gene III
    Expression
    Bacterial
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242139-plasmid-map-sequence-id-486013

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  3. pKS1466

    Plasmid
    #239516
    Purpose
    Express Idi1p and Erg20WW for synthesis of GPP in the peroxisome; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    ScErg20p(F96W_N127W)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239516-plasmid-map-sequence-id-475876

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  4. pKS1137

    Plasmid
    #239498
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239498-plasmid-map-sequence-id-475872

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  5. pKS1135

    Plasmid
    #239497
    Purpose
    Express synthetic sfCherry3C small fragment membrane protein cargo and peroxisomal mTagBFP2
    Depositor
    John Dueber
    Insert
    Ost1ss-sfCherry3C(11)-Pex3_TMD(+/-6AA)-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239497-plasmid-map-sequence-id-475868

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  6. pKS1169

    Plasmid
    #239503
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239503-plasmid-map-sequence-id-475865

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  7. pKS1025

    Plasmid
    #239496
    Purpose
    Express inactive peroxisomal RsAS as negative control
    Depositor
    John Dueber
    Insert
    Ubi-Y-RsAS(H360E)-Venus-ePTS1
    Expression
    Yeast
    Mutation
    H360E
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239496-plasmid-map-sequence-id-475861

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  8. pAIP4_3S60

    Plasmid
    #234231
    Purpose
    Heterologous protein expression of lectin OAA in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3S60
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234231-plasmid-map-sequence-id-464330

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  9. pAIP4_5M2Y

    Plasmid
    #234193
    Purpose
    Heterologous protein expression of B7LG64_ECO55 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5M2Y
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234193-plasmid-map-sequence-id-464158

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  10. pAIP4_3H6Q

    Plasmid
    #234185
    Purpose
    Heterologous protein expression of macrocypin-1a in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3H6Q
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234185-plasmid-map-sequence-id-464150

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  11. pAIP4_4Q2S

    Plasmid
    #234177
    Purpose
    Heterologous protein expression of C20G4.08 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4Q2S
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234177-plasmid-map-sequence-id-464142

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  12. pAIP4_4O6G

    Plasmid
    #234175
    Purpose
    Heterologous protein expression of uncharacterized protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4O6G
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234175-plasmid-map-sequence-id-464140

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  13. pAIP4_3K1H

    Plasmid
    #234157
    Purpose
    Heterologous protein expression of O25709_HELPY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3K1H
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234157-plasmid-map-sequence-id-464123

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  14. PT7-alphaHL

    Plasmid
    #227650
    Purpose
    pT7-driven expression of alpha-hemolysin, a pore-forming protein in lipid bilayers.
    Depositor
    Richard Murray
    Insert
    alpha-hemolysin
    Use
    Synthetic Biology
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227650-plasmid-map-sequence-id-452398

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  15. pBD006

    Plasmid
    #201306
    Purpose
    D16: Promoter of TraesCS5D02G320700 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D16: Promoter of TraesCS5D02G320700
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    201306-plasmid-map-sequence-id-409554

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  16. pBD004

    Plasmid
    #201304
    Purpose
    D14: Promoter of TraesCS3D02G368800 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D14: Promoter of TraesCS3D02G368800
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    201304-plasmid-map-sequence-id-409541

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  17. pBD005

    Plasmid
    #201305
    Purpose
    D15: Promoter of TraesCS3D02G517500 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D15: Promoter of TraesCS3D02G517500
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201305-plasmid-map-sequence-id-409522

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  18. pPD1023 TRE3GV DsRedExpress2 in TU5

    Plasmid
    #139257
    Purpose
    TRE3GV promoter and DsRedExpress2 in TUPV5
    Depositor
    Joshua Leonard
    Insert
    DsRedExpress2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139257-plasmid-map-sequence-id-325370

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  19. C77m

    Plasmid
    #120989
    Purpose
    MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AmpR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120989-plasmid-map-sequence-id-236056

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  20. C78m

    Plasmid
    #120990
    Purpose
    MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ChlorR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120990-plasmid-map-sequence-id-236059

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Showing: 1061 - 1080 of 3816 results