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Plasmid#165570PurposeSignificantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.DepositorInsertP45
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Lox-Stop-Lox TOPO Hygro
Plasmid#35688DepositorInsertloxP-STOP-loxP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOpen-THEAQpolA
Plasmid#165512PurposeDNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing.DepositorInsertTaq DNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 9, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMVlac-dirTopo-AU1-aGFP
Plasmid#61971PurposeA directional cloning vector of PCR-amplified cDNAs for mammalian expressionDepositorInsertslacZ alpha
EGFP
UseTagsExpressionMammalianMutationIt contains silent mutationsPromoterAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC-dirTopo-P2A-aGFP
Plasmid#61974PurposeA directional cloning vector of PCR-amplified cDNAs for mammalian expressionDepositorInsertslacZ alpha
EGFP
UseTagsExpressionMammalianMutationIt contains silent mutationsPromoterAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC-dirTopo-AU1-aGFP
Plasmid#61973PurposeA directional cloning vector of PCR-amplified cDNAs for mammalian expressionDepositorInsertslacZ alpha
EGFP
UseTagsExpressionMammalianMutationIt contains silent mutationsPromoterAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-SRM (sortase recognition motif)-hphMX4
Plasmid#54488PurposePCR based C-terminal SRM tagging in yeastDepositorInsertSRM (sortase-recognition motif)
UseYeast genome targetingTagshphMX4ExpressionMutationPromoterAvailable sinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
L0-T15 BpiI-p35s-BpiI
Plasmid#219698PurposeLevel 0 to insert p35S promoter to history-dependent targetDepositorInsertBpiI-p35s-BpiI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMVlac-dirTopo-P2A-aGFP
Plasmid#61972PurposeA directional cloning vector of PCR-amplified cDNAs for mammalian expressionDepositorInsertslacZ alpha
EGFP
UseTagsExpressionMammalianMutationIt contains silent mutationsPromoterAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTargetAmp
Plasmid#228552PurposeTemplate for PCR amplification for gRNA cloning plasmid by in vivo cloning.DepositorInsertAmpicillin resistance
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-SRM (sortase recognition motif)-kanMX6
Plasmid#54489PurposePCR based C-terminal SRM tagging in yeastDepositorInsertSRM (sortase-recognition motif)
UseYeast genome targetingTagskanMX6ExpressionMutationPromoterAvailable sinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 9bp dist
Plasmid#234131PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
UseTagsExpressionYeastMutationIntron truncationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 12bp dist
Plasmid#234130PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
UseTagsExpressionYeastMutationIntron truncationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 27bp dist
Plasmid#234129PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
UseTagsExpressionYeastMutationIntron truncationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 15bp dist
Plasmid#234128PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
UseTagsExpressionYeastMutationIntron truncationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 42bp dist
Plasmid#234127PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
UseTagsExpressionYeastMutationIntron truncationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 50bp dist
Plasmid#234112PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
UseTagsExpressionYeastMutationIntron truncationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 46bp dist
Plasmid#234111PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
UseTagsExpressionYeastMutationIntron truncationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
L0-T11 Circuit 2 GG4-PhiC31B-Bxb1B-GG6
Plasmid#219694PurposeLevel 0 to construct history-dependent target T2BPDepositorInsertGG4-PhiC31B-Bxb1B-GG6
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T12 GG2-ER-tagBFP-tUBQ10-GG3
Plasmid#219695PurposeLevel 0 to construct history-dependent targetDepositorInsertGG2-ER-tagBFP-tUBQ10-GG3
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only