We narrowed to 118,477 results for: cloning
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Plasmid#135455PurposeBacterial expression of uTEV1Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
TagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, S153N mutation…Promotertac promoterAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5
Plasmid#135455PurposeBacterial expression of uTEV1Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
TagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, S153N mutation…Promotertac promoterAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6HuR
Plasmid#135943PurposeExpresses HuR in E. coliDepositorAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6HuR
Plasmid#135943PurposeExpresses HuR in E. coliDepositorAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)
Plasmid#128607PurposeExpresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsert36xMashTag
ExpressionMammalianPromoterPcmvAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)
Plasmid#128607PurposeExpresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsert36xMashTag
ExpressionMammalianPromoterPcmvAvailable SinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTREX GPR68_NB2_2
Plasmid#127691PurposePlasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.DepositorInsertGPR68_NB2_2
ExpressionMammalianAvailable SinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTREX GPR68_NB2_2
Plasmid#127691PurposePlasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.DepositorInsertGPR68_NB2_2
ExpressionMammalianAvailable SinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV-Sox10MCS5-GFP-EF1a-mCherry
Plasmid#115782PurposeSox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.DepositorInsertsmouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
EF1alpha promoter driven constitutive mCherry
UseLentiviralTagsEF1α promoter fused to mCherry and cfos promoter …PromoterSOX10 Multiple Species Conserved enhancer element…Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV-Sox10MCS5-GFP-EF1a-mCherry
Plasmid#115782PurposeSox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.DepositorInsertsmouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
EF1alpha promoter driven constitutive mCherry
UseLentiviralTagsEF1α promoter fused to mCherry and cfos promoter …PromoterSOX10 Multiple Species Conserved enhancer element…Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-v2 RsTAL At4CL
Plasmid#126524Purposeexpresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesisDepositorInsertsTagsHisExpressionBacterialPromoterT7Available SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-v2 RsTAL At4CL
Plasmid#126524Purposeexpresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesisDepositorInsertsTagsHisExpressionBacterialPromoterT7Available SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGT-B1
Plasmid#122579PurposeMAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)DepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGT-B1
Plasmid#122579PurposeMAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)DepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-30-His6-SUMO-AEY-CfaN
Plasmid#122949PurposeBacterial expression of N-inteinDepositorInsertCfaN
ExpressionBacterialAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-30-His6-SUMO-AEY-CfaN
Plasmid#122949PurposeBacterial expression of N-inteinDepositorInsertCfaN
ExpressionBacterialAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
STING-V2
Plasmid#124264PurposeC-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
STING-V2
Plasmid#124264PurposeC-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_ETV2_P2A_Hygro_Barcode
Plasmid#120436PurposeBarcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_ETV2_P2A_Hygro_Barcode
Plasmid#120436PurposeBarcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only