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R989-X29-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V

Plasmid

#159556

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V

Depositor

Dominic Esposito

Publication

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

G12V

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X31-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

Plasmid

#159558

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

Depositor

Dominic Esposito

Publication

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

Q61L

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJEP320-pAAV-U6SaCas9gRNA(CREB3)_EFS-GFP-KASH-pA

Plasmid

#113697

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Publication

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTN-Y117C-FL

Plasmid

#110219

Purpose

Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Publication

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Tags

His6 and ZB

Expression

Bacterial

Mutation

Y117C, P296L (see depositor comments below)

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti_MS2_P65_HSF1_2A_puro

Plasmid

#118699

Purpose

To express MS2-P65-HSF1

Depositor

Christopher Vakoc

Publication

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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HA-MCPIP1

Plasmid

#127459

Purpose

Expressing human MCPIP1 in mammalian cells

Depositor

Mingui Fu

Publication

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-soCoChR-mCardinal

Plasmid

#107713

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. hSynapsin promoter

Depositor

Edward Boyden

Publication

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-mCardinal

Use

AAV

Tags

KA2 and mCardinal

Expression

Mammalian

Promoter

hSyn

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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F40 tension sensor

Plasmid

#101252

Purpose

Expresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.

Depositor

Carsten Grashoff

Publication

Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

Insert

YPet(short)-F40-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Promoter

CMV

Available since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMX-IY HA-GBRPL2

Plasmid

#89298

Purpose

For expression of the mammalian Atg8 protein GABARAPL2 tagged with HA

Depositor

Michael Lazarou

Publication

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Retroviral

Tags

HA and IRES-YFP

Expression

Mammalian

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a_Pro-IL-1α

Plasmid

#214341

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Publication

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL1A (IL1A Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+6)

Plasmid

#199160

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Publication

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+6)

Tags

MGHHHHHHGGA

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pWL078 MCP-2xGFP

Plasmid

#197047

Purpose

pCYC-NLS-MCP-2xGFP on pRS305

Depositor

Robert Singer

Publication

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

MCP-2xGFP

Expression

Yeast

Promoter

CYC1

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK2-S

Plasmid

#172426

Purpose

Expression of full-length, human GIRK2 with C-terminal StrepTag II

Depositor

Arthur Laganowsky

Publication

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK2 (KCNJ6 Human)

Tags

Streptag II

Expression

Insect

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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AF1521-c002

Plasmid

#173109

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))

Tags

mCerulean

Expression

Bacterial

Mutation

KH-M1-L192

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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ARH3-c002

Plasmid

#173095

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRS (ADPRS Human)

Tags

mCerulean

Expression

Bacterial

Mutation

A14-S363

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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MDO1-c014

Plasmid

#173103

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD1 (MACROD1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

S58-A325, codon optimized

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Parp14-c012

Plasmid

#173099

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP14 (PARP14 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

W1003-A1190

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Gi-c001

Plasmid

#173078

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GNAI1 (GNAI1 Human)

Tags

mCitrine

Expression

Bacterial

Mutation

codon optimized

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Parp14-c011

Plasmid

#173098

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP14 (PARP14 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

K791-F978

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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PARP9-c020

Plasmid

#173097

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP9 (PARP9 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

N310-N493

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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