We narrowed to 11,048 results for: ENA;

1. pSLIK-IDH1-R132H-FLAG

   Plasmid

   #66803

   Purpose

   Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector

   Depositor

   Christian Metallo

   Article

   Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

   Insert

   IDH1 (IDH1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R132H

   Promoter

   CMV

   Available Since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pStreptagII 3C EGFR EABR

   Plasmid

   #234994

   Purpose

   For production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminus

   Depositor

   Daniel Leahy, Monica Gonzalez-Magaldi

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   EGFR-EABR (EGFR Human)
   

   Use

   Tags

   Streptag-II, HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-PHFF-sh-hBVRA

   Plasmid

   #100285

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Inserts

   MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
   shRNA for human BVRA
   

   Use

   Tags

   FLAG, T7 and Mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   All genes are codon-optimized for expression in h…

   Promoter

   CAG promoter and H1 promoter

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Fibronectin-human-EDA in pMAX

   Plasmid

   #120402

   Purpose

   enables eukaryotic expression of human EDA (or EIIIA) containing fibronectin based on plasma fibronectin sequence

   Depositor

   Inaam Nakchbandi

   Article

   Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

   Insert

   EDA-fibronectin (FN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-10XHis-GST-HRV-dL5

   Plasmid

   #73214

   Purpose

   Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

   Insert

   EGFR (EGFR Human, Schistosoma japonicum)
   

   Use

   Tags

   10XHis-GST-HRV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pScalps_Puro_mTet2 catalytic domain HxD

   Plasmid

   #79611

   Purpose

   Expression of catalytically inactive mouse Tet2

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Insert

   Tet2 (Tet2 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mutation

   Mutant Tet2 with H1302Y, D1304A substitutions in …

   Promoter

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. Fibronectin-human-EDB in pMAX

   Plasmid

   #120403

   Purpose

   enables eukaryotic expression of human EDB (or EIIIB) containing fibronectin based on plasma fibronectin sequence

   Depositor

   Inaam Nakchbandi

   Article

   Kraft et al J Mol Med (Berl). 2016 May;94(5):567-81.

   Insert

   EDB-fibronectin (FN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-IDH2-R172K

   Plasmid

   #87927

   Purpose

   Expresses IDH2 (R172K mutant) in mammalian cells

   Depositor

   Reproducibility Project Cancer Biology

   Article

   Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

   Insert

   isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   R172K

   Promoter

   CMV

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

   Plasmid

   #208038

   Purpose

   Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   PML (isoform IVa) (PML Human)
   

   Use

   Lentiviral

   Tags

   FLAG, N-terminal Split-TurboID

   Expression

   Mutation

   Promoter

   EFS

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-3xFlag-dCas13-mTET2HxDCD

    Plasmid

    #228235

    Purpose

    For targeted tethering of an inactive mutant (HxD) of the catalytic domain of mouse TET2 using dCas13

    Depositor

    Chuan He

    Article

    Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-

    Inserts

    dCas13
    TET2 (Tet2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    CD (cysteine-rich, dioxygenase domain) truncation…

    Promoter

    CAG

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. hSyn-HA-PGRN-LAMP1TM-IRES-GFP

    Plasmid

    #234877

    Purpose

    Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.

    Depositor

    Andrew Arrant

    Article

    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

    Insert

    Progranulin (GRN Human)
    

    Use

    Lentiviral

    Tags

    HA tag and LAMP-1 transmembrane domain and cytoso…

    Expression

    Mammalian

    Mutation

    Promoter

    hSYn

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

    Plasmid

    #114078

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST

    Plasmid

    #208047

    Purpose

    Enables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Lentiviral

    Tags

    BirAopt

    Expression

    Mutation

    Cys>Ala mutations in conserved RING domain

    Promoter

    EFS

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRetroX-TRE3G-PLOD2_PGK-GpNLuc

    Plasmid

    #136457

    Purpose

    Expresses Tet/Dox-inducible human PLOD2 in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

    Insert

    TRE3G::PLOD2-T2A-Hygromycin (PLOD2 Human)
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCI-TPI_WT-IL6_3UTR-xrRNA-4H

    Plasmid

    #108373

    Purpose

    Expresses wild type TPI reporter; contains partial IL6 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type TPI reporter with partial IL6 control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, IL6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-PHFF-sh-mBVRA

    Plasmid

    #100286

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for mouse BVRA
    

    Use

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFR gRNA (BRDN0001146570)

    Plasmid

    #75688

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EGFR

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFR (EGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CMV-CASANOVA

    Plasmid

    #113036

    Purpose

    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EGFR gRNA (BRDN0001149231)

    Plasmid

    #75690

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EGFR

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFR (EGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFR gRNA (BRDN0001149464)

    Plasmid

    #75691

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EGFR

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFR (EGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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