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pUC19_CoV-2_frD ΔS/GFP

Plasmid

#253144

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of spike, GFP is inserted at the locus of Spike

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD ΔS/GFP

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_XBB_ΔE67 3*

Plasmid

#253122

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a, premature stop codon in orf3a at AA213. GFP is inserted in the locus of env

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Fragment D2 SARS-CoV-2 ΔEΔ6Δ7 orf3*

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_ΔE/GFP Δ68

Plasmid

#253121

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of envelope (residual AA) , orf6, orf8. GFP is inserted in the locus of envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Fragment D2 SARS-CoV-2 ΔE-GFPΔ6Δ8

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_S Omicron BA.2

Plasmid

#253117

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), Wuhan backbone with Omicron BA.2 spike; chimeric

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

SARS-CoV-2 Omicron BA.2 spike

Expression

Bacterial

Available Since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD2_ΔE67 3* NA-P2A-N PR8

Plasmid

#253162

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza N1 (WSN)-P2A-N

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with Influenza N1 PR8

Expression

Bacterial

Available Since

May 6, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_Δ3E_RABVG Δ67a

Plasmid

#253177

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; rabies glycoprotein replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with RABVG

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_Δ3E_GP LCMV Δ67a

Plasmid

#253179

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of orf3a, envelope, orf6, orf7a; LCMV glycoprotein replaces orf3a and envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD with LCMV GP

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔrE/esxA Δ67 3*

Plasmid

#253180

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Mycobacterium tuberculosis esxA replaces envelope

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with esxA

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD2_ΔE67 3*

Plasmid

#253186

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD2_ΔE67 ORF3*

Expression

Bacterial

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_S Omicron BA.1

Plasmid

#253116

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), Wuhan backbone with Omicron BA.1 spike; chimeric

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

SARS-CoV-2 Omicron BA.1 spike

Expression

Bacterial

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD2_ΔE67 3* VSVG-P2A-N

Plasmid

#253170

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein fused to N, P2A

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with VSV-G

Expression

Bacterial

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pQGG005

Plasmid

#231332

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI-ELT4 counterselectable site for BpiI cloning. L2-P15A-Gm-ISceI-ELT4; GG assembly from pOGG006, pOGG009, pNDGG006, pGQ0023, pOGG014. GmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-B/c

Plasmid

#227963

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

B/c

Expression

Plant

Promoter

35S

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-AtmiR173aTS-B/c

Plasmid

#227964

Purpose

Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
AtmiR173aTS

Promoter

None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-NbmiR482aTS-B/c

Plasmid

#227966

Purpose

Entry plasmid with NbmiR482aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
NbmiR482aTS

Promoter

None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMBS-859

Plasmid

#218009

Purpose

mCerulean fluorescent protein fused to Factor Xa Protease cleavage site and Strep-Tag II

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mCerulean

Use

Synthetic Biology

Tags

Factor Xa protease cleavage site and Strep-tag II

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-852

Plasmid

#218010

Purpose

Receptor binding domain of SARS-CoV-2

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

RBD (S SARS-CoV-2)

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-864

Plasmid

#218018

Purpose

GST-tag fused to TEV Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

GST-tag

Use

Synthetic Biology

Tags

TEV protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-845

Plasmid

#218000

Purpose

Strep-Tag II fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Strep-tag II

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-846

Plasmid

#218001

Purpose

3xFLAG-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

3xFLAG-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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