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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,551 results for: emb

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Showing: 10881 - 10900 of 17551 results

  2. pUC19-K1LM04

    Plasmid
    #103119
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    K1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103119-plasmid-map-sequence-id-201919

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  3. pChlr-8SE

    Plasmid
    #52172
    Purpose
    Encodes module 8 with amino acids 12S-16E for recognition of nt 1G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in module 8
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pChlr-4SYR

    Plasmid
    #52158
    Purpose
    Encodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S, 13H to 13Y, and 16Q to 16R in…
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pChlr-5SR

    Plasmid
    #52162
    Purpose
    Encodes module 5 with amino acids 12S-16R for recognition of nt 4C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16R in Module 5
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pChlr-6SE

    Plasmid
    #52164
    Purpose
    Encodes module 6 with amino acids 12S-16E for recognition of nt 3G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 6
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pBB0323-TEV-His12

    Plasmid
    #137039
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137039-plasmid-map-sequence-id-291948

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  9. pUC19-E6I4Q5

    Plasmid
    #103101
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103101-plasmid-map-sequence-id-201810

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  10. pJ136

    Plasmid
    #29486
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    May 17, 2011
    Availability
    Academic Institutions and Nonprofits only

  12. PR594-His-bio

    Plasmid
    #52332
    Purpose
    Expresses full-length Neuron-specific protein family member 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PR594 (NSG1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52332-plasmid-map-sequence-id-81052

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  13. EGFP-uTEV1-MAVS-mTA

    Plasmid
    #170999
    Purpose
    HiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Article
    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[R537A]
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[R537A]
    Promoter
    pTRE-tight (TetOn)
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170999-plasmid-map-sequence-id-335667

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  18. pChlr-4SE

    Plasmid
    #52152
    Purpose
    Encodes module 4 with amino acids 12S-16E for recognition of nt 5G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 4
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pChlr-8SR

    Plasmid
    #52174
    Purpose
    Encodes module 8 with amino acids 12S-16R for recognition of nt 1C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 8
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pChlr-7CQ

    Plasmid
    #52167
    Purpose
    Encodes module 7 with amino acids 12C-16Q for recognition of nt 2A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12S to 12C and 16E to 16Q in Module 7
    Available Since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 10881 - 10900 of 17551 results