We narrowed to 15,981 results for: LEA

1. pQE30-His-RF3

   Plasmid

   #124131

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Release factor 3 (prfC Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK792

   Plasmid

   #8830

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   TEV protease, S219P mutant
   

   Tags

   His, MBP (cleaved), and polyarginine

   Expression

   Bacterial

   Mutation

   S219P mutation

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b-His-RF2

   Plasmid

   #124130

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Release factor 2 (prfB Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ920

   Plasmid

   #42234

   Depositor

   Jennifer Doudna

   Article

   Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   GFP, HA epitope, and NLS (nuclear localization si…

   Expression

   Mammalian

   Mutation

   codon-optimized synthetic DNA sequence

   Promoter

   CMV

   Available Since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLBac-Bat-FluA-trimer

   Plasmid

   #229858

   Purpose

   Influenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)

   Depositor

   Stephen Cusack

   Article

   Pflug et al Nature. 2014 Dec 18;516(7531):355-60. do

   Inserts

   PA_Bat-FluA_AFC35437.1
   PB1_Bat-FluA_AFC35436
   PB2_Bat-FluA_AFC35435.1
   

   Expression

   Insect

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. PARG-c002

   Plasmid

   #173107

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARG (PARG Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   S448-T976

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-CalfluxCTN

   Plasmid

   #190068

   Purpose

   Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

   Depositor

   Carl Johnson

   Article

   Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

   Insert

   CalfluxCTN
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-OTI-TCRA_p2_TCRB

   Plasmid

   #131033

   Purpose

   Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

   Depositor

   Jeff Hammerbacher

   Article

   Aksoy et al bioRxiv

   Inserts

   TCRA (Tcra Mouse)
   TCRB (Tcrb Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV/T7

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. SBE-NLS-mCherry-P2A-CreERT2 PGK-rtTA3

   Plasmid

   #196936

   Purpose

   TGFbeta reporter driven nuclear mCherry and CreERT2 with PGK driven rtTA3

   Depositor

   Elaine Fuchs

   Article

   Oshimori et al Cell. 2015 Feb 26;160(5):963-976. doi: 1

   Insert

   mCherry
   

   Use

   Lentiviral and RNAi

   Tags

   P2A-CreERT2

   Expression

   Mammalian

   Promoter

   SBE

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST14-DoubleCatcher

    Plasmid

    #216284

    Purpose

    Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWT35S-mCherry-NLS

    Plasmid

    #194049

    Purpose

    Transient expression of mCherry-NLS in plant cell (Nucleus)

    Depositor

    Yutaka Kodama

    Article

    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

    Insert

    mCherry
    

    Tags

    SV40 (Nuclear localization signal)

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCW01

    Plasmid

    #72300

    Purpose

    Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Type

    Empty backbone

    Use

    Derivative of puc19

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHB-3

    Plasmid

    #140956

    Purpose

    For PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Expression

    Bacterial

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS42H_PTEF1_dCas9_TCYC1

    Plasmid

    #177155

    Purpose

    Expresses dCas9 in yeast cells

    Depositor

    Gautam Dantas

    Article

    Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

    Insert

    Nuclease-null Cas9
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Promoter

    TEF1 promoter

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR4-HA-myc-nuc (w557-1)

    Plasmid

    #20025

    Purpose

    Gateway entry vector for nuclear targeting of domains (N-term HA , C-term NLS, myc tags)

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    HA, Myc, and NLS

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-EF1aL-nlsGFP-W

    Plasmid

    #126688

    Purpose

    Constitutive, ubiquitous, nuclear-localized GFP expression.

    Depositor

    Darrell Kotton

    Article

    Tang et al Nat. Phys. 18, 1371–1378 (2022).

    Insert

    nlsGFP
    

    Use

    Lentiviral

    Tags

    6xHIS and NLS

    Promoter

    EF1aL

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO_HaloTag9_LamB1

    Plasmid

    #175538

    Purpose

    Expression of HaloTag9 at the nuclear lamina of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag9-LamB1
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO_Lyn11-HaloTag9

    Plasmid

    #175543

    Purpose

    Expression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lyn11-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. NES-mCherry-P4Mx1

    Plasmid

    #108143

    Purpose

    PI4P biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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