We narrowed to 15,822 results for: LEA

1. UAS-Syt::7xHalo7

   Plasmid

   #87650

   Purpose

   For creating drosophila transgenics expressing UAS-Syt::7xHalo7

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   Syt::7xHalo7
   

   Use

   Tags

   Expression

   Insect

   Mutation

   S265G in Syt (please see depositor's commen…

   Promoter

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MXS_P2A

   Plasmid

   #62433

   Purpose

   MXS_chaining vector with P2A

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   Self-cleaving peptide
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-FAK30

   Plasmid

   #59131

   Purpose

   Cloning vector for FAK fret sensor with 30 nm helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Use

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC-TALO8

   Plasmid

   #83409

   Purpose

   TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeast

   Depositor

   Toshi Tsukiyama

   Article

   Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

   Insert

   TRP-ARS-8xLacO
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Please see depositor comments below

   Promoter

   TRP1

   Available since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. csr-1_son-1_mTagBFP2

   Plasmid

   #191746

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and mTagBFP2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MECR

   Plasmid

   #38850

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MECR (Mecr Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pNCS Citrine

   Plasmid

   #91761

   Purpose

   Bacterial expression of a fluorescent protein, Citrine

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

   Insert

   Citrine
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA-FRT-FAK10

   Plasmid

   #59119

   Purpose

   FAK fret sensor with 10 nm helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Use

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGBW-m4046203

   Plasmid

   #145488

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCS2-FAK0

    Plasmid

    #59128

    Purpose

    Cloning vector for FAK fret sensor without helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJOG824

    Plasmid

    #105401

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    SV40 nuclear localization signal
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI/ BpiI restriction sites removed

    Promoter

    Available since

    March 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEG476

    Plasmid

    #40198

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (S404A) (mex-5 Nematode)
    

    Use

    Tags

    Dendra2 PAFP (sequence optimized for worm express…

    Expression

    Worm

    Mutation

    S404A

    Promoter

    pie-1

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCMV10-3xFLAG-prs-MCP1

    Plasmid

    #184341

    Purpose

    Express yeast MCP1 in Expi293F cells

    Depositor

    Karin Reinisch

    Article

    Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

    Insert

    Yeast MCP1 (MCP1 Budding Yeast)
    

    Use

    Tags

    3xFLAG-PreScission cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVITRO1-BFP-LaminB1

    Plasmid

    #184460

    Purpose

    Nuclear membrane-targeted EBFP2 expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    EBFP2-LaminB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. csr-1_son-1_vsfGFP9

    Plasmid

    #191747

    Purpose

    Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    NCU04288 (NCU04288 Neurospora crassa)
    

    Use

    Tags

    NCU04502 promoter and vsfGFP-9

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CBX4

    Plasmid

    #25237

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX4_02 (CBX4 Human)
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pUE1-TSP-UL32

    Plasmid

    #162658

    Purpose

    Expresses UL32 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    UL32
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE30-TEV-pro-conA

    Plasmid

    #159527

    Purpose

    Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.

    Depositor

    Joshua Mylne

    Article

    Nonis et al bioRxiv. 2020.

    Insert

    Canavalia ensiformis pro-concanavalin A
    

    Use

    Tags

    Six-Histidine tag and tobacco etch virus (TEV) pr…

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDN3.1(+)-CaNARi

    Plasmid

    #64848

    Purpose

    FRET-based calcineurin (CaN) activation biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Use

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRATIO2214

    Plasmid

    #141303

    Purpose

    Gateway cloning compatible dual fluorescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Use

    Tags

    mCerulean and mScarlet-I

    Expression

    Plant

    Mutation

    Promoter

    UBQ10 promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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