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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 5461 - 5470 of 18063 results

  6. pET27b+R

    Plasmid
    #188245
    Purpose
    Empty backbone vector for protein expression in Escherichia coli and his-tag purification
    Depositor
    Yiping Qi
    Article
    Fang et al Methods Mol Biol. 2023;2653:153-171. doi
    Type
    Empty backbone
    Tags
    6xHis tag and TEV site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188245-plasmid-map-sequence-id-368371

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  7. pJB75

    Plasmid
    #226320
    Purpose
    E. coli protein expression for protein purification
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    6xHis-(Y to A MR1-6)-strep
    Tags
    6xHis and Strep
    Expression
    Bacterial
    Mutation
    N-terminal domain deleted, C-terminal domain deleā€¦
    Promoter
    T7
    Available Since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    226320-plasmid-map-sequence-id-449984

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  8. pJB72

    Plasmid
    #226312
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with Y to A CsoS2 MR1
    Expression
    Bacterial
    Mutation
    Y300A
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226312-plasmid-map-sequence-id-449924

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  9. pJB44

    Plasmid
    #226303
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with C to S MR1
    Expression
    Bacterial
    Mutation
    C292S, C310S
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226303-plasmid-map-sequence-id-449915

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  10. pJB43

    Plasmid
    #226302
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    WT CsoS2, frameshift site intact
    Expression
    Bacterial
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226302-plasmid-map-sequence-id-449914

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Showing: 5461 - 5470 of 18063 results