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pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

Plasmid

#229823

Purpose

A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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MT2

Plasmid

#223330

Purpose

Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Publication

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

3HA-TurboID-EGFP-OMP25 (Synj2bp Rat)

Tags

3HA-TurboID-EGFP

Expression

Plant

Promoter

CaMV35S

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pME nls EGFP WPRE bGH pA (JDW 488)

Plasmid

#224540

Purpose

A Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleus

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nls-EGFP-WPRE-bGH-pA

Use

Gateway Cloning

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-puro SNAP-CD86-eGFP-FKBP(f36v)

Plasmid

#200283

Purpose

Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP reporter under Tetracycline-inducible promoter

Depositor

Kristina Ganzinger

Publication

Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

Insert

SNAPtag-eGFP-CD86tm-eGFP-FKBP(f36v)

Use

Lentiviral

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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MT3

Plasmid

#223331

Purpose

Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

Depositor

Xiaohong Zhu

Publication

Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

Insert

3HA-EGFP-miniTurboID-OMP25 (Synj2bp Rat)

Tags

3HA-EGFP-miniTurboID

Expression

Plant

Promoter

CaMV35S

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC25A46[R247A,R347A]-His (SB257)

Plasmid

#227606

Purpose

Inducible expression of His-tagged SLC25A46 with R247A and R347A mutations in Pichia pastoris

Depositor

Luke Chao

Publication

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

10xHis

Expression

Yeast

Mutation

R247A,R347A

Promoter

AOX1

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[R340C]-P2A-Electra1 (pAH12)

Plasmid

#227617

Purpose

Overexpression of SLC25A46 with R340C mutation in mammalian cell lines

Depositor

Luke Chao

Publication

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

R340C

Promoter

CMV, T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[I349D]-P2A-Electra1 (pAH13)

Plasmid

#227618

Purpose

Overexpression of SLC25A46 with I349D mutation in mammalian cell lines

Depositor

Luke Chao

Publication

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

I349D

Promoter

CMV, T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

Plasmid

#224505

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexOP-35SCaMV

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (C159A/C219A/C365A/C399A)

Plasmid

#224754

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A/C365A/C399A)

Depositor

Dan Minor

Publication

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

C159A/C219A/C365A/C399A

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28(Y191F)

Plasmid

#223628

Purpose

Lentiviral expression of human CD28 (Y191F) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Mutation

Y191F

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD28(Y191F)

Plasmid

#223623

Purpose

Lentiviral expression of human CD28 (Y191F) with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Mutation

Y191F

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD86-SNAP

Plasmid

#223613

Purpose

Lentiviral expression of human CD86 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyA-GFP

Plasmid

#220189

Purpose

sgRNA expression vector - pEM7 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pEM7 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pEM7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyD-GFP

Plasmid

#220192

Purpose

sgRNA expression vector - pBG28 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG28 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG28

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-GFP

Plasmid

#220197

Purpose

mini-Tn7 GFP expression vector

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

promoterless

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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LBR(R583Q)-FKBP-GFP

Plasmid

#222423

Purpose

To express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Publication

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR(R583Q) (LBR Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

R583Q

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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LBR(N547D)-FKBP-GFP

Plasmid

#222422

Purpose

To express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Publication

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR(N547D) (LBR Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

N547D

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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MRGPRF-DuET

Plasmid

#213342

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Publication

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

MRGPRF (MRGPRF Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

Plasmid

#179717

Purpose

Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

Depositor

Edwin Chapman

Publication

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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