We narrowed to 15,822 results for: LEA

1. UAS-Syt::3xHalo7

   Plasmid

   #87649

   Purpose

   For creating drosophila transgenics expressing UAS-Syt::3xHalo7

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   Syt::3xHalo7
   

   Use

   Tags

   Expression

   Insect

   Mutation

   S265G in Syt (please see depositor's commen…

   Promoter

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. CHIKV-nsP3-c001

   Plasmid

   #173086

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   N/A
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   A1334-T1493, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4046494

   Plasmid

   #149137

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 surface glycoprotein (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. CLAPon

   Plasmid

   #213405

   Purpose

   Peroxidase (APEX) reporter for TEV protease cleavage

   Depositor

   Wenjing Wang

   Article

   Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

   Insert

   E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJY3948

   Plasmid

   #113338

   Purpose

   6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

   Depositor

   Suzanne Sandmeyer

   Article

   Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

   Insert

   2-pyrone synthase, codon optimized for Y. lipolytica
   

   Use

   Tags

   6XHis

   Expression

   Yeast

   Mutation

   Please see depositor comments

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pISceI-myl7:MBIC5-mCer3

   Plasmid

   #74117

   Purpose

   Zebrafish transgenic construct

   Depositor

   Michael Tsang

   Article

   He et al Nat Methods. 2016 Mar;13(3):263-268. doi

   Insert

   MBIC5-mCer3
   

   Use

   Mrna generation for microinjection.

   Tags

   mCerulean 3

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046373

   Plasmid

   #145555

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAPV-DsRed2-FLAG-ER-5

   Plasmid

   #170622

   Purpose

   Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   APV-DsRed2-ER-5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

   Plasmid

   #175522

   Purpose

   Nuclear expression of HaloTag11 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid

    #69548

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Expression

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. NAT-TET-OFF -LEU- 4xFLAG

    Plasmid

    #182527

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    7 Copies of TetO Box
    

    Use

    Tags

    4XFLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. RECQL5 (RECQL5A-c011)

    Plasmid

    #98231

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RECQL5A (RECQL5 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…

    Promoter

    T7

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHelper_O

    Plasmid

    #173783

    Purpose

    The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    186 Integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    E59G - please see depositor comments

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046464

    Plasmid

    #148970

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. human KANK1 Ankyrin Repeats pET151

    Plasmid

    #164053

    Purpose

    Expresses human KANK1 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. 5FAI (EMG1)

    Plasmid

    #79519

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EMG1 (EMG1 Human)
    

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    June 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAAV_TALEN-FokI(WT)-BB_V3

    Plasmid

    #42588

    Depositor

    Feng Zhang

    Article

    Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

    Insert

    TALEN-FokI(WT)-BB
    

    Use

    AAV; Adeno-associated virus ()

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG hybrid

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCellFree_G03 NFX1

    Plasmid

    #67131

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    NFX1 (NFX1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAS1-rab-3P::his-24::GCaMP6s

    Plasmid

    #124351

    Purpose

    Nuclear Localized Pan-Neuronal GCaMP6s

    Depositor

    Andrew Leifer

    Article

    Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

    Insert

    rab-3, GCaMP6s (rab-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICSL50016

    Plasmid

    #50315

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Codon-optimised for A. thaliana. BsaI/BbsI sites …

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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