We narrowed to 15,982 results for: LEA

1. pcDNA5-H2B_Halo_T2A_EGFP

   Plasmid

   #135444

   Purpose

   Mamalian cell expression of nuclear HaloTag7 and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

   Insert

   H2B-HaloTag7 and EGFP
   

   Tags

   H2B

   Expression

   Mammalian

   Promoter

   CMV/TetO

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. KLHL40 (KBTBD5A-c006)

   Plasmid

   #98233

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KBTBD5A (KLHL40 Human)
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-N1-jGCaMP7b-XN

   Plasmid

   #178362

   Purpose

   Nuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.

   Depositor

   Xiaodong Liu

   Article

   Geng et al eLife. 2022 Oct 5;11:e76691. doi: 10.755

   Insert

   jGCaMP7b-XN
   

   Expression

   Mammalian

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRGD-TcR

   Plasmid

   #74110

   Purpose

   Plasmid containing tetracycline resistance gene cassette flanked by multiple restriction sites

   Depositor

   Hans-Martin Fischer

   Article

   Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

   Insert

   tetA(C)
   

   Use

   Bacterial vector based on pmb1 replicon

   Mutation

   C-to-T transition at pos. no. 1049 of tetA(C) lea…

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. NES-mTagBFP2-P4Mx1

   Plasmid

   #108142

   Purpose

   PI4P biosensor

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   X.leavis map2k1.L(32-44):mTagBFP2:L. penomophila SidM(546-647)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 14UAS zfPSD95:GFP

   Plasmid

   #74314

   Purpose

   A fusion of zebrafish PSD95 to GFP under control of 14 UAS repeats

   Depositor

   Martin Meyer, Stephen Smith

   Article

   Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

   Insert

   zebrafish PSD95
   

   Tags

   EGFP

   Mutation

   Y599H and I793S (please see depositor comment bel…

   Promoter

   UAS

   Available Since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pET21a - NR

   Plasmid

   #89625

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Nitrate reductase
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV10-3xFLAG-prs-XK

   Plasmid

   #184369

   Purpose

   Express human XK in Expi293 cells

   Depositor

   Karin Reinisch

   Article

   Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

   Insert

   Human XK (XK Human)
   

   Tags

   3xFLAG-PreScission cleavage site

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Parp14-c011

   Plasmid

   #173098

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   K791-F978

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TARG1-c002

    Plasmid

    #173108

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    TARG1
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    Full length protein

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5KO9 (HMCES)

    Plasmid

    #101877

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HMCES (HMCES Human)
    

    Tags

    6x His (modified C-term tag is AENLYFQSHHHHHH)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. UtB2N7

    Plasmid

    #161015

    Purpose

    ubiquitously expressed nuclear localized tagBFP2

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    tagBFP2
    

    Expression

    Plant

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBA2675

    Plasmid

    #189997

    Purpose

    Inducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)

    Depositor

    Bungo Akiyoshi

    Article

    Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do

    Inserts

    FBP75
    VhhGFP4
    

    Use

    Expression vector for trypanosoma brucei

    Tags

    NLS

    Mutation

    N-terminal 600 bp of FBP75

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. zCD2

    Plasmid

    #122031

    Purpose

    Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

    Insert

    Zebrafish HDAC6 Catalytic Domain 2
    

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. csr-1_son-1_mNeonGreen

    Plasmid

    #191743

    Purpose

    Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    NCU04288 (NCU04288 Neurospora crassa)
    

    Tags

    NCU04502 promoter and mNeonGreen

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-GFP-P2A

    Plasmid

    #80807

    Purpose

    GFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptide

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    GFP-P2A
    

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. VKG1-gRNA-pX330

    Plasmid

    #108671

    Purpose

    Cleavage of VKG1 sequence by CRISPR/Cas9

    Depositor

    Shigeo Sugano

    Article

    Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

    Insert

    gRNA for VKG1 sequence
    

    Use

    CRISPR

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETNb-nALFA

    Plasmid

    #159988

    Purpose

    For the protein expression with ALFA tag at N-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Tags

    MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

    Expression

    Bacterial

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSCW02

    Plasmid

    #72301

    Purpose

    Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Type

    Empty backbone

    Use

    Derivative of puc19

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET26b_Nb.AT110.i1

    Plasmid

    #135481

    Purpose

    E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

    Depositor

    Andrew Kruse

    Article

    Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

    Insert

    Nanobody AT110.i1
    

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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