Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 12,203 results for: LEA

Clear keywords
Showing: 11061 - 11080 of 12203 results

  4. pAvA143

    Plasmid
    #248147
    Purpose
    mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutations
    Depositor
    Emil Jensen
    Publication
    van Aalst et al Commun Biol. 2025 Nov 27. doi: 10.1038/s
    Insert
    luxR (gen3 mutations)
    Use
    Integration vector
    Tags
    Gal4 activation domain and nuclear localization s…
    Expression
    Yeast
    Mutation
    Gal4_AD: F51 (TTC→TTT), G88E. LuxR: K53E, N86Y, I…
    Promoter
    pPGK1
    Available since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248147-plasmid-map-sequence-id-497172

    Enlarge

  5. pSquash2_SFFV-eGFP-NR3C1-IRES-mCherry_NeomycinR

    Plasmid
    #247927
    Purpose
    Overexpression vector for eGFP tagged GR or NR3C1. Monitor post-translational degradation of GR via EGFP:mCherry ratio. Contains neomycin-resistance selection marker.
    Depositor
    Benjamin Ebert, Jonathan Tsai
    Publication
    Tsai et al Mol Cell. 2023 Aug 3;83(15):2753-2767.e1
    Insert
    Glucocorticoid Receptor (NR3C1 Human)
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247927-plasmid-map-sequence-id-496528

    Enlarge

  7. pHBT-NRAS174

    Plasmid
    #248051
    Purpose
    To express His6 tag-Avi tag-TEV cleavage site-NRAS (residues 1-174) in E. coli, under the T7 promoter.
    Depositor
    Shohei Koide
    Publication
    Wallon et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Insert
    NRAS (NRAS Human)
    Tags
    His6 tag-Avi tag-TEV cleavage site
    Expression
    Bacterial
    Mutation
    includes aa 1-174 only
    Promoter
    T7
    Available since
    Nov. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    248051-plasmid-map-sequence-id-493230

    Enlarge

  10. pUC-GW-Kan-VPC

    Plasmid
    #244163
    Purpose
    Reporter for human p38alpha activation state by FRET
    Depositor
    Thomas Kuhlman
    Insert
    MAPK14/p38a (MAPK14 Human)
    Use
    Synthetic Biology
    Tags
    mCerulean3 and mVenus
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Sept. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    244163-plasmid-map-sequence-id-487534

    Enlarge

  11. pBIRC5-Split PS Intein-N tTA

    Plasmid
    #242033
    Purpose
    N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the BIRC5 promoter.
    Depositor
    Vladislav Verkhusha
    Publication
    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:
    Insert
    Split PS Intein-N tTA
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    The CMV promoter was replaced with the BIRC5 prom…
    Promoter
    hTERT
    Available since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242033-plasmid-map-sequence-id-483131

    Enlarge

  12. phTERT-Split PS Intein-N tTA

    Plasmid
    #242034
    Purpose
    N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the hTERT promoter.
    Depositor
    Vladislav Verkhusha
    Publication
    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:
    Insert
    Split PS Intein-N tTA
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    The CMV promoter was replaced with the hTERT prom…
    Promoter
    hTERT
    Available since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242034-plasmid-map-sequence-id-481812

    Enlarge

  14. pEJS2785 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-125a CMV-BFP

    Plasmid
    #240515
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Publication
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-125a Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240515-plasmid-map-sequence-id-481869

    Enlarge

  15. pEJS2786 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-151a CMV-BFP

    Plasmid
    #240516
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Publication
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-151a Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240516-plasmid-map-sequence-id-477188

    Enlarge

  16. pEJS2787 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-221 CMV-BFP

    Plasmid
    #240517
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Publication
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-221 Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240517-plasmid-map-sequence-id-477189

    Enlarge

Showing: 11061 - 11080 of 12203 results