We narrowed to 16,144 results for: LEA
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Plasmid#34586DepositorInsertNLS-LSS-mKate2
UseRetroviralExpressionMammalianMutationK69Y/P131T/S148G/M167D/T183S/M196V compared to mK…Available SinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-⍺-Lock
Plasmid#216286PurposeExpresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ⍺-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-β-Lock
Plasmid#216287PurposeExpresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher β-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-γ-Lock
Plasmid#216288PurposeExpresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher γ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-δ-Lock
Plasmid#216289PurposeExpresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher δ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-ε-Lock
Plasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
JAK3 (JAK3A-c023)
Plasmid#98229PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJAK3A (JAK3 Human)
UseBaculovirusTagsHis-6-TEVMutationpfam00069:Pkinase 822-1091PromoterpolyhedrinAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMVMagFRET-1-NLS
Plasmid#50748PurposeExpresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.DepositorInsertgene encoding for MagFRET-1 with NLS
ExpressionMammalianAvailable SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHB-2
Plasmid#140955PurposeFor PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.DepositorInsertphaCAB
ExpressionBacterialMutationR238S mutation in PhaCAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK30
Plasmid#59121PurposeFAK fret sensor with 30 nm helixDepositorInsertFocal Adhesion Kinase
TagsFLAG, mCerulean, and mCitrineExpressionMammalianPromoterCMVAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAD6
Plasmid#134349PurposeE. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tagDepositorAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS1938
Plasmid#82563PurposeHigh copy number plasmid containing Ple261 (CLDN5 MiniPromoter) insert.DepositorInsertPle261
UsePleiades promoter project [sic, pleaides plieades]Available SinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
6CCR (GID4)
Plasmid#119960PurposeBacterial expression for structure determinationDepositorInsertGID4 (C17orf39 Human)
Tags6xHIS, thrombin,SUMO and TEV cleavageExpressionBacterialMutationcontains amino acids 116-300PromoterT7Available SinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBNb-cALFA
Plasmid#159990PurposeFor the protein expression with ALFA tag at C-terminus in insect cellsDepositorTypeEmpty backboneTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionInsectAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDA_449
Plasmid#179093Purposenuclease Cas expressionDepositorInsertSpG
UseCRISPR and LentiviralAvailable SinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF mito-cGFP (matrix)
Plasmid#188904PurposeExpresses mitochondrial GFPDepositorInsertmitochondrial GFP
TagsMitochondrial leader sequence: MSVLTPLLLRGLTGSARR…ExpressionMammalianAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
KLHL3 (KLHL3A-c003)
Plasmid#110251PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJCC_083 Cas12i1
Plasmid#171670PurposeFor bacterial expression of Cas12i1 with an N-terminal His-MBP tagDepositorInsertCas12i1
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
KEAP1 (KEAP1A-c029)
Plasmid#110247PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
DOT1L
Plasmid#36196PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDOT1L (GeneScript Synthesized) (DOT1L Human)
TagsHis tag with TEV cleavageExpressionBacterialMutationcontains amino acid residues 1-420Available SinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits