We narrowed to 16,272 results for: LEA
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Plasmid#216284PurposeExpresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked bindersDepositorInsertDoubleCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHB-3
Plasmid#140956PurposeFor PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.DepositorInsertphaCAB
ExpressionBacterialAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCS Citrine
Plasmid#91761PurposeBacterial expression of a fluorescent protein, CitrineDepositorInsertCitrine
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-lacO-His-sfGFP
Plasmid#180754PurposepET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
Tagsstrep tagExpressionBacterialMutationG140C- Please see depositor commentsAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_HaloTag9_LamB1
Plasmid#175538PurposeExpression of HaloTag9 at the nuclear lamina of the mammalian cellsDepositorInsertHaloTag9-LamB1
ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDN3.1(+)-CaNARi
Plasmid#64848PurposeFRET-based calcineurin (CaN) activation biosensor.DepositorInsertCaNARi
TagsCerulean and VenusExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-mCherry-NLS
Plasmid#194049PurposeTransient expression of mCherry-NLS in plant cell (Nucleus)DepositorInsertmCherry
TagsSV40 (Nuclear localization signal)ExpressionPlantPromoterCaMV 35S promoterAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCW01
Plasmid#72300PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19ExpressionBacterialPromoterlacAvailable SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKCalpha.FLAG
Plasmid#10805DepositorInsertPKC alpha (PRKCA Bovine)
TagsFLAGExpressionMammalianMutationD332G-please see comments belowAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-PP
Plasmid#154247Purposebackbone with PreScission protease-cleavable N-terminal OneStrepFLAG tagDepositorTypeEmpty backboneTagsFLAG and Strep-Tag IIExpressionMammalianAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalPromoterTgTUB1 and Toxoplasma U6Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-mCherry-LaminB1
Plasmid#186348PurposeNuclear membrane-targeted mCherry expression in mammalian cellsDepositorInsertmCherry-LaminB1
ExpressionMammalianMutationWTPromoterRat EF1αAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP1-10_pcDNA3.1(-)
Plasmid#182931PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragmentDepositorTypeEmpty backboneTags3C-cleavable split GFP 1-10, FLAG tag, and HA sig…ExpressionMammalianPromoterCMVAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lyn11-HaloTag7
Plasmid#175533PurposeExpression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cellsDepositorInsertLyn11-HaloTag7
ExpressionMammalianAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS42H_PTEF1_dCas9_TCYC1
Plasmid#177155PurposeExpresses dCas9 in yeast cellsDepositorInsertNuclease-null Cas9
TagsSV40 NLSExpressionYeastPromoterTEF1 promoterAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNH-NanoLuc
Plasmid#173075PurposeExpression vector for E. coli producing target protein with N-terminal Nanoluciferase fusionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
UAS-Syt::7xHalo7
Plasmid#87650PurposeFor creating drosophila transgenics expressing UAS-Syt::7xHalo7DepositorInsertSyt::7xHalo7
ExpressionInsectMutationS265G in Syt (please see depositor's commen…Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-myc-nuc (w556-1)
Plasmid#20026PurposeGateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)DepositorTypeEmpty backboneUseGateway entry vectorTagsMyc and NLSExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP
Plasmid#175521PurposeNuclear expression of HaloTag10 in mammalian cellsDepositorInsertH2B-HaloTag10
ExpressionMammalianMutationHaloTag10 = HaloTag7-Q165WAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-iGlu3Fast
Plasmid#226500PurposeUltrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate releaseDepositorInsertiGluSnFR3 S72T
TagsIgK, Myc tag, and PDGFR Beta TM domainExpressionMammalianMutationS72TPromoterCMVAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only