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pBAD-luxKOk

Plasmid

#229944

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKOk fusion bioluminescent probe (max. Em=562nm)

Depositor

Andy Yeh

Publication

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKOk

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-luxGold

Plasmid

#229943

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

Depositor

Andy Yeh

Publication

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxGold

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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aTY65_pTRE_Wnt3a_IRES_tagBFP2_WPRE

Plasmid

#225349

Purpose

Lentiviral vector for Wnt3a and BFP expression

Depositor

Wendell Lim

Publication

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Wnt3a (Wnt3a Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMS18

Plasmid

#225681

Purpose

Confers puromycin resistance to Salpingoeca rosetta

Depositor

Thibaut Brunet

Publication

Combredet et al bioRxiv 2024.07.13.603360

Insert

pEFL-Pac-Actin_3'UTR

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJJB1402

Plasmid

#218616

Purpose

Express Pex15, Pex22, Pex4, Pex1, and Pex6

Depositor

John Dueber

Publication

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex15

Expression

Yeast

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1578

Plasmid

#218620

Purpose

Express Pex22, Pex6, Pex15, and Pex8

Depositor

John Dueber

Publication

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex22

Expression

Yeast

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pEY139

Plasmid

#223486

Purpose

tph-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tph-1 (tph-1 Nematode)

Expression

Worm

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEY121

Plasmid

#223468

Purpose

sra-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

sra-6 (sra-6 Nematode)

Expression

Worm

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEY112

Plasmid

#223459

Purpose

oig-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

oig-1 (oig-1 Nematode)

Expression

Worm

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSX6

Plasmid

#90468

Purpose

U6 wt in pSE358 (TRP1 CEN)

Depositor

Christine Guthrie

Publication

Madhani et al Genes Dev. 1990 Dec;4(12B):2264-77.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Promoter

native

Available since

June 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1_CylLS_CylM

Plasmid

#208760

Purpose

Expresses modified cytolysin S (mCylLS) in E. coli

Depositor

Wilfred van der Donk

Publication

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CylLS
CylLM

Tags

Hisx6 Tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Ac5-STABLE1-blast

Plasmid

#208033

Purpose

Enables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Publication

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pO-066

Plasmid

#195435

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF1

Depositor

Ahmad Khalil

Publication

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF1 BS - minCMV - mCherry

Use

Lentiviral

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEY18

Plasmid

#191024

Purpose

flp-19 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-19 (flp-19 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY29

Plasmid

#191034

Purpose

gcy-36 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-36 (gcy-36 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY64

Plasmid

#191061

Purpose

aqp-6 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

aqp-6 (aqp-6 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY49

Plasmid

#191053

Purpose

lim-6 fluorescent neural reporter driving nuclear mNeptune2.5 and CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

lim-6 (lim-6 Nematode)

Expression

Worm

Mutation

None

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_136

Plasmid

#180520

Purpose

Empty backbone for D1.1 cloning, contains sfGFP dropout

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_001

Plasmid

#180517

Purpose

Empty backbone for creation of entry parts for the KTK, contains sfGFP dropout

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pYCTK027

Plasmid

#176731

Purpose

The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Saccharomyces cerevisiae (receiver part).

Depositor

Victor Sourjik

Publication

Krink et al bioRxiv 2021

Insert

ste2Sc

Use

Synthetic Biology

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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