We narrowed to 16,272 results for: LEA
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Plasmid#191743PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
TagsNCU04502 promoter and mNeonGreenExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
14UAS zfPSD95:GFP
Plasmid#74314PurposeA fusion of zebrafish PSD95 to GFP under control of 14 UAS repeatsDepositorInsertzebrafish PSD95
TagsEGFPMutationY599H and I793S (please see depositor comment bel…PromoterUASAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
UtB2N7
Plasmid#161015Purposeubiquitously expressed nuclear localized tagBFP2DepositorInserttagBFP2
ExpressionPlantAvailable SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-GFP-P2A
Plasmid#80807PurposeGFP without stop codon followed by porcine teschovirus-1 2A (P2A) "self-cleaving" peptideDepositorInsertGFP-P2A
Available SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECFP-DEVG-Venus
Plasmid#24538DepositorInsertECFP-DEVG-Venus
TagsECFP and VenusExpressionMammalianMutationDEVDR mutated to DEVGPromoterCMVAvailable SinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
FnR9-10
Plasmid#182567PurposeHuman fibronectin fragment, type-III repeats 9-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 9-10
TagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379AAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopTag-AffiHER2-SpyTag
Plasmid#216280PurposeExpresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expressionDepositorInsertSnoopTag-AffiHER2-SpyTag
UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, SpyTag, and Thrombin cleavage sit…ExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AT110.i1
Plasmid#135481PurposeE. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptorDepositorInsertNanobody AT110.i1
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GR-AKAR3
Plasmid#173015PurposeImproved green-red FRET-based PKA activity reporter.DepositorInsertGR-AKAR3
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-NLS-LSSmKate2
Plasmid#34586DepositorInsertNLS-LSS-mKate2
UseRetroviralExpressionMammalianMutationK69Y/P131T/S148G/M167D/T183S/M196V compared to mK…Available SinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NR
Plasmid#89625PurposeExpresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG inductionDepositorInsertNitrate reductase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSCW02
Plasmid#72301PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19ExpressionBacterialPromoterlacAvailable SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMVMagFRET-1-NLS
Plasmid#50748PurposeExpresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.DepositorInsertgene encoding for MagFRET-1 with NLS
ExpressionMammalianAvailable SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
VKG1-gRNA-pX330
Plasmid#108671PurposeCleavage of VKG1 sequence by CRISPR/Cas9DepositorInsertgRNA for VKG1 sequence
UseCRISPRAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK30
Plasmid#59121PurposeFAK fret sensor with 30 nm helixDepositorInsertFocal Adhesion Kinase
TagsFLAG, mCerulean, and mCitrineExpressionMammalianPromoterCMVAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBA2675
Plasmid#189997PurposeInducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)DepositorInsertsFBP75
VhhGFP4
UseExpression vector for trypanosoma bruceiTagsNLSMutationN-terminal 600 bp of FBP75Available SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
JAK3 (JAK3A-c023)
Plasmid#98229PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJAK3A (JAK3 Human)
UseBaculovirusTagsHis-6-TEVMutationpfam00069:Pkinase 822-1091PromoterpolyhedrinAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBNb-cALFA
Plasmid#159990PurposeFor the protein expression with ALFA tag at C-terminus in insect cellsDepositorTypeEmpty backboneTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionInsectAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-⍺-Lock
Plasmid#216286PurposeExpresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ⍺-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-β-Lock
Plasmid#216287PurposeExpresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher β-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only