We narrowed to 15,981 results for: LEA

1. pDGE62

   Plasmid

   #79444

   Purpose

   recipient plasmid [single nuclease]; p35S:Cas9, nptII

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMH-HT_RcCHAD

   Plasmid

   #127782

   Purpose

   Expresses RcCHAD in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   RcCHAD
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4046217

   Plasmid

   #149071

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. ANKRA2

   Plasmid

   #36904

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ANKRA2 (ANKRA2 Human)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   amino acid residues 148-313 (ANK repeats)

   Available Since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pRDA_381

   Plasmid

   #179092

   Purpose

   nuclease Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   IDT-Cas9_v2
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDA_157

   Plasmid

   #179090

   Purpose

   nuclease Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   HypaCas9
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRDA_156

   Plasmid

   #179089

   Purpose

   nuclease Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   Cas9-VRER
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDA_155

   Plasmid

   #179088

   Purpose

   nuclease Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   Cas9-VQR
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_154

   Plasmid

   #179087

   Purpose

   nuclease Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   xCas9-3.7
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDA_153

    Plasmid

    #179086

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    evoCas9
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_152

    Plasmid

    #179085

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    eCas9-1.1
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDA_151

    Plasmid

    #179084

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    Cas9-HF
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBW-m4046581

    Plasmid

    #149064

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGBW-m4046589

    Plasmid

    #145450

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGBW-m4046249

    Plasmid

    #148991

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCS2-FAK20

    Plasmid

    #59130

    Purpose

    Cloning vector for FAK fret sensor with 20 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBW-m4046268

    Plasmid

    #145423

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pGBW-m4046216

    Plasmid

    #145490

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGBW-m4046286

    Plasmid

    #149147

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 surface glycoprotein (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. AASS (AASSA-c013)

    Plasmid

    #98227

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AASSA (AASS Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Mutation

    pfam03435:Saccharop_dh 483-916

    Promoter

    polyhedrin

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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