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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,144 results for: LEA

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Showing: 11141 - 11160 of 16144 results

  1. pJY3948

    Plasmid
    #113338
    Purpose
    6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression
    Depositor
    Suzanne Sandmeyer
    Article
    Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.
    Insert
    2-pyrone synthase, codon optimized for Y. lipolytica
    Tags
    6XHis
    Expression
    Yeast
    Mutation
    Please see depositor comments
    Available Since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113338-plasmid-map-sequence-id-218737

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  4. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid
    #69548
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69548-plasmid-map-sequence-id-122974

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  5. pGBW-m4046373

    Plasmid
    #145555
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145555-plasmid-map-sequence-id-384616

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  6. RECQL5 (RECQL5A-c011)

    Plasmid
    #98231
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RECQL5A (RECQL5 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…
    Promoter
    T7
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98231-plasmid-map-sequence-id-193411

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  7. pAPV-DsRed2-FLAG-ER-5

    Plasmid
    #170622
    Purpose
    Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-ER-5
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170622-plasmid-map-sequence-id-335778

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  10. 5FAI (EMG1)

    Plasmid
    #79519
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EMG1 (EMG1 Human)
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79519-plasmid-map-sequence-id-150117

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  12. pGBW-m4046464

    Plasmid
    #148970
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available Since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    148970-plasmid-map-sequence-id-297202

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  18. human KANK1 Ankyrin Repeats pET151

    Plasmid
    #164053
    Purpose
    Expresses human KANK1 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164053-plasmid-map-sequence-id-327672

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  19. pSDMA66

    Plasmid
    #128355
    Purpose
    gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.
    Depositor
    James Fraser
    Article
    Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do
    Insert
    Noureothricin (NAT)
    Use
    Unspecified
    Promoter
    ACT1
    Available Since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    128355-plasmid-map-sequence-id-254590

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  20. pGBW-m4258326

    Plasmid
    #154793
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 surface glycoprotein (Spike protein) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154793-plasmid-map-sequence-id-311545

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Showing: 11141 - 11160 of 16144 results