We narrowed to 15,981 results for: LEA

1. pCY 3010-07

   Plasmid

   #36212

   Depositor

   Anne Robinson

   Article

   Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

   Insert

   Streptoalloteichus hindustanus bleomycin (Sh ble)
   

   Use

   Yeast cassette for pcr and homologous recombinati…

   Tags

   yEpolylinker-Cerulean-Zeocin

   Available Since

   June 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. ACVR1 (ACVR1Z-c041)

   Plasmid

   #110269

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ACVR1 (ACVR1 Human)
   

   Tags

   His-TEV

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMTB-Multibow-hfR

   Plasmid

   #60998

   Purpose

   Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. . Encodes nuclear mKate2.

   Depositor

   Sean Megason

   Article

   Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

   Insert

   mKate2
   

   Use

   Zebrafish

   Tags

   histone 2B tag

   Promoter

   Bactin2

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

4. MTK4a_002

   Plasmid

   #123816

   Purpose

   Encodes Nuclear Export sequence as a Type 4a part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   NES
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK3a_002

   Plasmid

   #123755

   Purpose

   Encodes Nuclear Export sequence as a Type 3a part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   NES
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK3a_003

   Plasmid

   #123756

   Purpose

   Encodes Nuclear import sequence as a Type 3a part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   NLS
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046380

   Plasmid

   #148977

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Tags

   Cleavable thrombin;MBP

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. HECW2

   Plasmid

   #32875

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HECW2 (HECW2 Human)
   

   Tags

   His tag (with TEV cleavage)

   Expression

   Bacterial

   Available Since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pDGE64

   Plasmid

   #79446

   Purpose

   recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MXS_H2B

    Plasmid

    #62432

    Purpose

    MXS_chaining vector with H2B

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Histone 2B with leading Kozak sequence
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PIWIL2

    Plasmid

    #32042

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PIWIL2 (PIWIL2 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    PAZ domain only (amino acids 386-533)

    Available Since

    Jan. 3, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pGBW-m4046194

    Plasmid

    #148979

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. EPB41

    Plasmid

    #32040

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EPB41 (EPB41 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    FERM domain only (amino acids 1-279)

    Available Since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. GHRHR

    Plasmid

    #39173

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GHRHR (GHRHR Human)
    

    Use

    Baculovirus expression

    Tags

    Signal-His6-TEV

    Mutation

    N20->Q mutation of putative glycosylsation sit…

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pGBW-m4252811

    Plasmid

    #153563

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHis-003

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pGBW-m4046548

    Plasmid

    #149130

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGBW-m4046386

    Plasmid

    #149089

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. mEos4a-LaminA-C-18

    Plasmid

    #57497

    Purpose

    Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Loren Looger, Michael Davidson

    Insert

    LaminA (LMNA Human)
    

    Tags

    mEos4a

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pGBW-m4046455

    Plasmid

    #145487

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. dFP

    Plasmid

    #120542

    Purpose

    Expresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.

    Depositor

    Brian Chow

    Article

    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

    Insert

    de novo fluorescence-activating protein (dFP)
    

    Tags

    6xHis - TEV cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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