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Showing: 1101 - 1120 of 8664 results

  1. pJK560

    Plasmid
    #71585
    Purpose
    Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with Arg232>His mutant (YfdE-R232H)
    Depositor
    T. J. Kappock
    Article
    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:
    Insert
    acetyl-CoA:oxalate CoA-transferase (yfdE )
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed arginine-232 to histidine
    Promoter
    T7 promoter
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71585-plasmid-map-sequence-id-130921

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  2. pJK504

    Plasmid
    #71584
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes cysteine-357 to tyrosine
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71584-plasmid-map-sequence-id-131755

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  3. pJK612

    Plasmid
    #72533
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72533-plasmid-map-sequence-id-132621

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  4. pJK613

    Plasmid
    #72534
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72534-plasmid-map-sequence-id-132623

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  5. pJK619

    Plasmid
    #72536
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72536-plasmid-map-sequence-id-132627

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  9. pJK274

    Plasmid
    #71564
    Purpose
    Produces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeast
    Depositor
    T. J. Kappock
    Article
    Nam et al J Exp Bot. 2007;58(6):1421-32. Epub 2007
    Insert
    delta12 fatty acid desaturase (form 3)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    GAL1 promoter
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71564-plasmid-map-sequence-id-129501

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  11. Galt1-Grx1-roGFP1-iE

    Plasmid
    #230029
    Purpose
    Expressing Grx1-roGFP1-iE fluorescent fusion protein in mammalian cells with localization in the Golgi apparatus.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Galt1-Grx1-roGFP1-iE (B4GALT1 Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230029-plasmid-map-sequence-id-458346

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  13. pRAM28

    Plasmid
    #234314
    Purpose
    pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications
    Depositor
    James Chappell
    Article
    Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103
    Insert
    U1367 Targeting RAM, Barcode 2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    P. CymRC
    Available since
    March 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234314-plasmid-map-sequence-id-466009

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  14. Galt1-sCGrx1p-HA

    Plasmid
    #230028
    Purpose
    Expressing sCGrx1p protein fused to HA tag in mammalian cells with localization in the Golgi apparatus.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Galt1-sCGrx1p-HA (B4GALT1 Human, Synthetic)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230028-plasmid-map-sequence-id-458168

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  15. BiP-mGFP

    Plasmid
    #62231
    Purpose
    Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.
    Depositor
    Erik Snapp
    Article
    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2
    Insert
    hamster BiP-mGFP-KDEL
    Use
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    62231-plasmid-map-sequence-id-314151

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  17. BiP-mCherry

    Plasmid
    #62233
    Purpose
    Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.
    Depositor
    Erik Snapp
    Article
    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2
    Insert
    hamster BiP-mCherry-KDEL
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    62233-plasmid-map-sequence-id-314153

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  18. CyTERM-mGFP

    Plasmid
    #62237
    Purpose
    ER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.
    Depositor
    Erik Snapp
    Article
    Costantini et al Traffic. 2012 May;13(5):643-9. doi: 10.1
    Insert
    CyTERM-mGFP
    Use
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    signal anchor of rabbit cytochrome p450 and monom…
    Promoter
    CMV
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    62237-plasmid-map-sequence-id-320621

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Showing: 1101 - 1120 of 8664 results