We narrowed to 1,127 results for: fret

1. pSBbi-Pur-Lyn-AktAR2-D

   Plasmid

   #125198

   Purpose

   SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter

   Depositor

   Eric Davis

   Article

   Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

   Insert

   Lyn-AktAR2-D
   

   Use

   Transposon

   Tags

   Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]

   Expression

   Mammalian

   Mutation

   FoxO1 Thr-24 changed to Val rendering this inacti…

   Promoter

   EF1a/RPBSA

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-YFP-KRAS4B

   Plasmid

   #112718

   Purpose

   Expresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.

   Depositor

   Kenneth Westover

   Article

   Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

   Insert

   YFP-KRAS4B (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-CFP-KRAS4B

   Plasmid

   #112717

   Purpose

   Expresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.

   Depositor

   Kenneth Westover

   Article

   Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

   Insert

   CFP-KRAS4B (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA5/FRT/TO_COX8-Halo-PanK

   Plasmid

   #197949

   Purpose

   Expression of FRET_control in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

   Insert

   Halo-ecPanK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PanK=PanK-D127A,L277W,F252Y

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA5/FRT/TO_Halo-PanK

   Plasmid

   #197948

   Purpose

   Expression of FRET_control in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

   Insert

   Halo-ecPanK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PanK=PanK-D127A,L277W,F252Y

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1(−) mCER-Prx2

   Plasmid

   #158224

   Purpose

   Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for transient transfection in mammalian cells)

   Depositor

   Tobias Dick

   Article

   Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

   Insert

   mCER-Prx2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-SUMO mCER-Prx2

   Plasmid

   #158226

   Purpose

   Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression)

   Depositor

   Tobias Dick

   Article

   Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

   Insert

   mCER-Prx2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLPCX mCER-Prx1

   Plasmid

   #158227

   Purpose

   Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)

   Depositor

   Tobias Dick

   Article

   Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

   Insert

   mCER-Prx1
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLPCX mCER-Prx2

   Plasmid

   #158225

   Purpose

   Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)

   Depositor

   Tobias Dick

   Article

   Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

   Insert

   mCER-Prx2
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

    Plasmid

    #189752

    Purpose

    Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mTurquoise2_spatial-linker-P2A_mScarlet3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

    Plasmid

    #189755

    Purpose

    Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mTurquoise2_spatial-linker-P2A_mScarlet-I3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNH13 (pmyo-2::QuasAr::mOrange)

    Plasmid

    #130273

    Purpose

    Expresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    QuasAr::mOrange
    

    Use

    Tags

    mOrange

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNH12 (pmyo-2::MacQ::mCitrine)

    Plasmid

    #130274

    Purpose

    Expresses the eFRET-based voltage sensor MacQ::mCitrine in the pharynx of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    MacQ::mCitrine
    

    Use

    Tags

    mCitrine

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

    Plasmid

    #123353

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-Cameleon
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-mCherry-mCherry-FLARE-AKAR

    Plasmid

    #123333

    Purpose

    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-AKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Venus-Venus-FLARE-Cameleon

    Plasmid

    #123351

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-Venus-FLARE-Cameleon
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-mCherry-mCherry-FLARE-Cameleon

    Plasmid

    #123352

    Purpose

    Red-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-Cameleon
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

    Plasmid

    #123346

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-CKAR
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-GFP-GFP-FLARE-AKAR

    Plasmid

    #123332

    Purpose

    Green-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    GFP-GFP-FLARE-AKAR
    

    Use

    Tags

    6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Venus-Venus-FLARE-AKAR

    Plasmid

    #123331

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-Venus-FLARE-AKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge