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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,317 results for: golden gate

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Showing: 1101 - 1120 of 5317 results

  14. C114m

    Plasmid
    #121013
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9 with no bbsI or BsaI sites
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121013-plasmid-map-sequence-id-245424

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  16. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    226429-plasmid-map-sequence-id-458099

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  17. pUAP1

    Plasmid
    #63674
    Purpose
    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.
    Depositor
    Nicola Patron
    Publication
    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np
    Insert
    RFP cloning selection cassette
    Use
    Synthetic Biology
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    63674-plasmid-map-sequence-id-106088

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Showing: 1101 - 1120 of 5317 results