We narrowed to 15,981 results for: LEA

1. pLV-REPORT(EF1a no GFP)

   Plasmid

   #172339

   Purpose

   mnucTomato

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL1_CSL3

   Plasmid

   #200173

   Purpose

   Amino acids 1-195 of CSL3 with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vector

   Depositor

   Michael Jewett

   Article

   Warfel et al Glycobiology. 2023 Mar 7:cwad018. doi: 1

   Insert

   CSL3
   

   Tags

   6x His tag and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL1_SUL-I

   Plasmid

   #200172

   Purpose

   Amino acids 25-308 of SUL-I with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vector

   Depositor

   Michael Jewett

   Article

   Warfel et al Glycobiology. 2023 Mar 7:cwad018. doi: 1

   Insert

   SUL-I
   

   Tags

   6x His tag and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. csr-1_son-1_sGFP

   Plasmid

   #191561

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Tags

   NCU04502 promoter and sGFP

   Expression

   Bacterial

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. csr-1_son-1_mRuby3

   Plasmid

   #191744

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Tags

   NCU04502 promoter and mRuby3

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. csr-1_son-1_mNeptune2.5

   Plasmid

   #191748

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Tags

   NCU04502 promoter and mNeptune2.5

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVRC8400-SARS-CoV-2-NTD-BA.4/5-AVI

   Plasmid

   #192113

   Purpose

   Expresses SARS-CoV-2 NTD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Insert

   SARS-CoV-2-NTD-BA.4/5 (S )
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. human KANK2-A670V Ankyrin Repeats pET151

   Plasmid

   #164055

   Purpose

   Expresses human KANK2 Ank domain - A670V mutant in bacterial cells

   Depositor

   Ben Goult

   Insert

   Kidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
   

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mouse KANK3 Ankyrin Repeats pET151

   Plasmid

   #164056

   Purpose

   Expresses mouse KANK3 Ank domain in bacterial cells

   Depositor

   Ben Goult

   Insert

   Kidney Ankyrin Repeat-Containing Protein 3 (Kank3 Mouse)
   

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mouse KANK4 Ankyrin Repeats pET151

    Plasmid

    #164057

    Purpose

    Expresses mouse KANK4 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. His8OphMA-21mer.

    Plasmid

    #189670

    Purpose

    His8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.

    Depositor

    Markus Künzler, James Naismith

    Article

    Emmanuel et al bioRxiv 2022.07.21.500988

    Insert

    His8OphMATEV18mer
    

    Tags

    His8 tagged

    Expression

    Bacterial

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. His8OphMADeltaC6-18mer.

    Plasmid

    #189671

    Purpose

    His8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptides

    Depositor

    Markus Künzler, James Naismith

    Article

    Emmanuel et al bioRxiv 2022.07.21.500988

    Insert

    His8OphMA∆C6-TEV18mer
    

    Tags

    His8 tagged

    Expression

    Bacterial

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVITRO1-TagRFP-LaminB1

    Plasmid

    #184448

    Purpose

    Nuclear memebrane-targeted TagRFP expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    TagRFP-LaminB1
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNUT2

    Plasmid

    #175745

    Purpose

    Single-component photo-inducible (LOV2-based) nucleolus-targeting tool 2

    Depositor

    Yubin Zhou

    Article

    Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655

    Insert

    mCherry-LOV2-NoLS (human nuclear factor-κB inducing kinase)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTS1018-Tier1-PhCMV-p2A

    Plasmid

    #169491

    Purpose

    Tier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven 2A sequence of porcine teschovirus-1
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVITRO1-mCardinal-LaminB1

    Plasmid

    #184452

    Purpose

    Nuclear memebrane-targeted mCardinal expression in mammalian cells

    Depositor

    Chuan-Hsiang Huang

    Article

    Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

    Insert

    mCardinal-LaminB1
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Rat EF1α

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. H2B-HT-mGold

    Plasmid

    #183988

    Purpose

    Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    H2B (H2BC11 Human)
    

    Tags

    HaloTag, mGold

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAPV-FLAG-DsRed2-ER-5

    Plasmid

    #170620

    Purpose

    Expresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-FLAG-DsRed2-ER-5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEPV-FLAG-DsRed2-ER-5

    Plasmid

    #170621

    Purpose

    Expresses FLAG-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    EPV-FLAG-DsRed2-ER-5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. EC0_3_Mxi1

    Plasmid

    #163715

    Purpose

    Plasmid encoding Mxi1 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    Mxi1+SV40 NLS
    

    Use

    Synthetic Biology; Moclo vector level 0 (type 4a)

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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