Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 16,144 results for: LEA

Showing: 11261 - 11280 of 16144 results

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pDest17_DBD

Plasmid

#231886

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 DBD

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 DBD

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WW+

Plasmid

#231885

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WW+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Swap1

Plasmid

#231879

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap1

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Swap1

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R3

Plasmid

#231874

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R3

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103R, D133R, K143R

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 P+

Plasmid

#231872

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 P+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 P+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S122P, T131F, A141P, E143D

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 K5

Plasmid

#231866

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 K5

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 K5

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103K, D115K, D127K, D133K, E143K

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 E3

Plasmid

#231863

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 E3

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D105E, D118E, D139E

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 W120A

Plasmid

#231862

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 W120A

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

W120A

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1035 (pME_V5_TCF7L1_P2A_H2A_mCherry)

Plasmid

#232125

Purpose

A middle entry gateway clone containing V5 tagged human TCFL7 / TCF3 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L1 / TCF3 (TCF7L1 Human)

Use

Gateway cloning

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Med15

Plasmid

#231887

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Med15

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Med15

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R3 W

Plasmid

#231876

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R3 W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R3 W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103R, N126W, D133R, K143R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R2

Plasmid

#231870

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

K118R, K140R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 L123A+

Plasmid

#231869

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 L123A+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L123A+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123A

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Swap2

Plasmid

#231865

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Swap2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 F124I

Plasmid

#231864

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 F124I

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F124I

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Swap3

Plasmid

#231860

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap3

Depositor

Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Swap3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 WT 44mer

Plasmid

#231856

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WT 44mer

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 44mer

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_FL Gcn4

Plasmid

#231855

Purpose

Bacterial expression of N-terminally 6His tagged FL Gcn4

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYB1-T4gp47

Plasmid

#228212

Purpose

Expression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

47 (47 Escherichia phage T4)

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-mScarlet-i

Plasmid

#191196

Purpose

Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & mScarlet-I fusion

Tags

6xHis and mScarlet-I

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Showing: 11261 - 11280 of 16144 results

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