163,409 results

1. pSico

   Plasmid

   #11578

   Purpose

   Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

   Depositor

   Tyler Jacks

   Article

   Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi_RP_2XMTS-MicroID2-FLAG

   Plasmid

   #188524

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   2XMTS-MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET/ELP-I-CAT

   Plasmid

   #71461

   Purpose

   ELP-intein purification system

   Depositor

   David Wood

   Article

   Banki et al Nat Methods. 2005 Sep;2(9):659-61.

   Type

   Empty backbone

   Use

   Tags

   ELP ((VPGXG)110) and intein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJC6-EV

   Plasmid

   #163454

   Purpose

   empty vector control for pLJC6

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pINTO-N3::hTET3

   Plasmid

   #136364

   Purpose

   Expresses epitope-tagged human TET3 WT in mammalian cells

   Depositor

   Roberto Bonasio

   Article

   Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

   Insert

   TET3 (TET3 Human)
   

   Use

   Tags

   2xStrep-tagII, FLAG, and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T-1 3xFlag-p38alpha

   Plasmid

   #47575

   Purpose

   Bacterial expression plasmid for GST-3xFlag-tagged p38alpha

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   p38alpha (Mapk14 Mouse)
   

   Use

   Tags

   3xFlag and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

7. IFI16-FL (pcDNA3-FLAG)

   Plasmid

   #35064

   Depositor

   Cheryl Arrowsmith

   Article

   Liao et al Structure. 2011 Mar 9;19(3):418-29.

   Insert

   IFI16 (IFI16 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-Flag-Tet2 CD

   Plasmid

   #72219

   Purpose

   Express flag-tagged mouse Tet2 catalytic domain.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 2 (Tet2 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET2 catalytic domain.

   Promoter

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pJBL7010

   Plasmid

   #136942

   Purpose

   Constitutive sfGFP expression (high), bacterial promoter

   Depositor

   Julius Lucks

   Article

   Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGE687

    Plasmid

    #167299

    Purpose

    Vector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. TNFR1-YFP

    Plasmid

    #111209

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Insert

    TNFRSF1A (TNFRSF1A Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Human J-chain

    Plasmid

    #158215

    Purpose

    Express the human J-chain

    Depositor

    Junyu Xiao

    Article

    Li et al Science. 2020 Feb 28;367(6481):1014-1017

    Insert

    JCHAIN (JCHAIN Human)
    

    Use

    Tags

    8×his

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. B-95.ΔA

    Bacterial Strain

    #197933

    Purpose

    BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

    Bacterial Resistance

    None

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

14. pCW-SPI1

    Plasmid

    #162824

    Purpose

    Doxycycline-inducible overexpression of human SPI1

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. G protein alpha-q-GFP

    Plasmid

    #66080

    Purpose

    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope

    Depositor

    Catherine Berlot

    Article

    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.

    Insert

    G protein alpha-q-EE-YFP (Gnaq Mouse)
    

    Use

    Tags

    GFP was inserted internally into the alpha-q sequ…

    Expression

    Mammalian

    Mutation

    BamHI and SacI sites in alpha-q were removed with…

    Promoter

    CMV

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Archon1-KGC-EGFP-ER2-WPRE

    Plasmid

    #108423

    Purpose

    Archon1 fluorescent voltage reporter in mammalian expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. peGFP NOVA2 op

    Plasmid

    #224354

    Purpose

    Express GFP-tagged human NOVA2 (codon optimized)

    Depositor

    Nicolas Charlet-Berguerand

    Article

    Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

    Insert

    NOVA2 (NOVA2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    codon optimized for expression in human cells

    Promoter

    CMV and T7

    Available since

    Sept. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. Jag1-HA pIRES

    Plasmid

    #17336

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2007 Dec 12. ():.

    Insert

    Jagged1 (Jag1 Rat)
    

    Use

    Tags

    HA X3

    Expression

    Mammalian

    Mutation

    A silent C->G mutation at 3237

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-mCherry-IRES-Cre

    Plasmid

    #55632

    Purpose

    Expresses Cre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV8

    Inserts

    mCherry
    Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a and Ef1a/IRES

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1/Ndi-1/myc-His

    Plasmid

    #127503

    Purpose

    Expresses myc-tagged yeast Ndi-1 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    Ndi-1 (NDI1 Budding Yeast)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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