167,601 results

1. pcDNA3.1(+)-HLA-Flag-natT1R3

   Plasmid

   #113950

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R3 (TAS1R3 Human)
   

   Tags

   FLAG and Signal/leader sequence from HLA class I …

   Expression

   Mammalian

   Mutation

   residues 21-852

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAS

   Plasmid

   #60847

   Purpose

   Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

   Depositor

   Jamie Cate

   Article

   Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

   Inserts

   S. pyogenese Cas9
   RNA pol III promoter (tRNA-Tyr)
   hepatitis delta virus ribozyme, genomic
   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   NLS/His8 and TTT 3' extension prior to sgRNA

   Expression

   Bacterial and Yeast

   Mutation

   L4 is UUCG tetraloop and guide targets LYP1 (CATA…

   Available Since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD_Kan-LacI

   Plasmid

   #79826

   Purpose

   Arabinose induced expression of Lac repressor

   Depositor

   Katja Arndt

   Article

   Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

   Insert

   LacI
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   araBp

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET259-pUC57 24xMS2V6

   Plasmid

   #104391

   Purpose

   MS2V6 cloning

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V6
   

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. GCH1_pLX307

   Plasmid

   #98336

   Purpose

   Lentiviral expression of GCH1

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   GCH1 (GCH1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-pro-Il1b-C-Flag

   Plasmid

   #75131

   Purpose

   Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   interleukin 1 beta (Il1b Mouse)
   

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   (please see depositor comments below)

   Promoter

   CMV

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEmCherry2

   Plasmid

   #118413

   Purpose

   Red fluorescent reporter for assessing CRISPR gRNA activity

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p4xSTAT6-Luc2P

   Plasmid

   #35554

   Depositor

   Axel Nohturfft

   Insert

   4xStat6 promoter
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   4xStat6

   Available Since

   March 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

   Plasmid

   #40810

   Depositor

   Natalie Ahn

   Article

   Mansour et al Science. 1994 Aug 12;265(5174):966-70.

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta(31-51), S218E, S222D

   Promoter

   CMV

   Available Since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA-Wnt3A

    Plasmid

    #35908

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt3A (WNT3A Human)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRE-Tn7-152

    Plasmid

    #118566

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDA_867

    Plasmid

    #231109

    Purpose

    A>G base editor

    Depositor

    John Doench, David Root

    Insert

    ABE8e-nCas9-SpG-HA
    

    Use

    CRISPR and Lentiviral

    Mutation

    D10A

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. p6599 MSCV-IP N-HAonly FOSL1

    Plasmid

    #34897

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    FOSL1 (FOSL1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pInducer20 ALPK1 Y254C

    Plasmid

    #231587

    Purpose

    ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    changed tyrosine 254 to cysteine

    Promoter

    doxycycline

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-Sec61b-C1

    Plasmid

    #90994

    Purpose

    Expresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mCherry-Sec61b (SEC61B Human, Synthetic)
    

    Tags

    mCherry (red fluorescence)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-Tre3G-29xGGGGCC-12xMS2

    Plasmid

    #99149

    Purpose

    Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector. *Please see comments section below about the number of verified repeats.

    Depositor

    Ron Vale

    Article

    Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

    Insert

    29xGGGGCC 12xMS2 hairpins
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1 EGFP-Tau

    Plasmid

    #108868

    Purpose

    Expresses EGFP linked to human Tau

    Depositor

    Gabriele Kaminski Schierle

    Insert

    Tau (MAPT Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    human four-repeat tau lacking the N-terminal sequ…

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

    Plasmid

    #182352

    Purpose

    Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. B-gTEMP/pcDNA3

    Plasmid

    #188447

    Purpose

    B-gTEMP for mammalian expression

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1+DPP6

    Plasmid

    #117272

    Purpose

    Encodes human DPP6 variant 1

    Depositor

    Mark Cannell

    Article

    Lainez et al Biochem Pharmacol. 2018 Apr;150:120-130.

    Insert

    DPP6 (DPP6 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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