167,601 results

1. cacnb2a (rat) in pMT2 vector

   Plasmid

   #107424

   Purpose

   Expresses Calcium channel beta2a auxillary subunit in mammalian cells

   Depositor

   Annette Dolphin

   Article

   Wyatt et al J Physiol. 1998 Jul 15;510 ( Pt 2):347-6

   Insert

   calcium channel beta2a auxillary subunit (Cacnb2 Rat)
   

   Expression

   Mammalian

   Promoter

   Ad MLP

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLHKI-pGFPN3

   Plasmid

   #21917

   Depositor

   Hossein Ardehali

   Article

   Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

   Insert

   Full length human HKI in pGFP-N3 (HK1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This is full length human HKI in pGFP-N3

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX458M-53BP1-DN1S

   Plasmid

   #131045

   Purpose

   Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.

   Depositor

   Punam Malik

   Article

   Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:

   Insert

   SpCas9-53BP1-DN1S (TP53BP1 Human)
   

   Use

   CRISPR

   Tags

   GFP and SpCas9

   Expression

   Mammalian

   Mutation

   Only expressing residues 1231-1644 of complete 53…

   Promoter

   CBH

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1-B-GRAM-W-NeoR

   Plasmid

   #211710

   Purpose

   Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W)

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Lentiviral

   Tags

   ddFP-B3

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Tryptophan

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Human Brunello kinome pooled library, guides 1-4 in lentiGuide-Puro backbone

   Pooled Library

   #75312

   Purpose

   Human sgRNA library in backbone lentiGuide-Puro targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pBA615

   Plasmid

   #186239

   Purpose

   eLbuCas13a Negative Control (RFP targeting)

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Insert

   pTet-eLbuCas13a + crRNA negative control
   

   Expression

   Bacterial

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV8)

   Viral Prep

   #55645-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent Channelrhodopsin-EYFP expression for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   Syn

   Tags

   EYFP (Cre- and Flp-dependent)

   Available Since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pAS165

   Plasmid

   #194488

   Purpose

   97-4 Op (Part 2)

   Depositor

   Ahmad Khalil

   Article

   Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

   Insert

   97-4 Op (Part 2)
   

   Use

   Synthetic Biology

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDD131

   Plasmid

   #119886

   Purpose

   Optimized aTc inducible pTet promoter for Rhodococcus opacus expressing mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

   Insert

   pTet_mCherry
   

   Expression

   Bacterial

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.1-YY1

    Plasmid

    #169018

    Purpose

    Expression of YY1 driven by CMV promoter

    Depositor

    Claes Wadelius

    Article

    Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

    Insert

    human YY1 (YY1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHM066

    Plasmid

    #225524

    Purpose

    Encodes Wnt-responsive destablized iRFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Insert

    iRFP
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV pCAG-FLEX-tdTomato-WPRE

    Plasmid

    #51503

    Purpose

    Can be used to generate AAV virus that will express tdTomato in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    tdTomato
    

    Use

    AAV

    Promoter

    pCAG

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-scFvGCN4sfGFPTET1CD

    Plasmid

    #82561

    Purpose

    Expression vector for antibody-sfGFP-TET1CD.

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    scFvGCN4sfGFPTET1CD
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-kanMX6

    Plasmid

    #39296

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE

    Plasmid

    #89609

    Purpose

    over-expression of VEGF-A(165) in human cells

    Depositor

    Fernando Fierro

    Insert

    VEGF-A(165) (VEGFA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    K188E

    Promoter

    MNDU3

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Orai1 YFP

    Plasmid

    #19756

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 (ORAI1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Oct. 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. BL21ΔF

    Bacterial Strain

    #102259

    Purpose

    ompF deletion strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    None

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

18. FKBP-CD28TMD-NTEVp(H75S)

    Plasmid

    #137828

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(H75S)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Histidine 75 to Serine in NTEVp fragment

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-3XFLAG-EGFP-OMP25

    Plasmid

    #83354

    Purpose

    For tagging mitochondria with Sigma FLAG epitopes

    Depositor

    David Sabatini

    Article

    Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

    Insert

    3XFLAG-EGFP-OMP25
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRC-APEX2

    Plasmid

    #72558

    Purpose

    Expression of cytosolic APEX2 in bacteria

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    A134P relative to APEX

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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