163,421 results

1. pCAG-HIF1 alpha

   Plasmid

   #21101

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_PGK_SNIPR_CD8alpha SNIPR

   Plasmid

   #188357

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. RCASBP-Y DV

   Plasmid

   #11478

   Depositor

   William Pavan

   Article

   Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

   Insert

   Gateway ccdB reading frame cassette
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-Tet-Puro-shIAPEz_2

   Plasmid

   #185017

   Purpose

   shRNA mediated knockdown

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   ERV_IAPEz
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-Tet-Puro-shIAPEz_1

   Plasmid

   #185016

   Purpose

   shRNA mediated knockdown

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   ERV_IAPEz
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGR481

   Plasmid

   #213783

   Purpose

   Integrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutations

   Depositor

   Chang Liu

   Insert

   ura3*
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. GPR161-Tango

   Plasmid

   #66334

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR161 (GPR161 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pSNAPf-hYTHDF1

   Plasmid

   #155346

   Purpose

   Express full-length human YTHDF1 with a C-terminal SNAP-tag in mammalian cells

   Depositor

   Xiaowei Zhuang

   Article

   Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

   Insert

   YTHDF1 (YTHDF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry

   Plasmid

   #114322

   Purpose

   Mifepristone-inducible lentiviral backbone for Gateway cloning of Gene insert together with constitutive mCherry expression

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. 1013 pcDNA3 T7 Akt1 T308A S473A

    Plasmid

    #9030

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 T308A S473A (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T308A S473A

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid

    #194469

    Purpose

    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

    Depositor

    Mathieu Boulard

    Article

    Formichetti et al Dev Cell 10 Jan 2024

    Insert

    BtGH84
    

    Use

    For in vitro transcription from t3 promoter after…

    Tags

    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTO135_pENO1-CaCas9-tCyc1

    Plasmid

    #171103

    Purpose

    Expresses Cas9 in Candida auris

    Depositor

    Teresa O'Meara

    Article

    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

    Insert

    CaCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO1

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pk7-GR-GFP

    Plasmid

    #15534

    Depositor

    Ian Macara

    Article

    Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

    Insert

    Glucocorticoid receptor (NR3C1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The glucocorticoid receptor was cloned into the X…

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid

    #177672

    Purpose

    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells

    Depositor

    Karl Deisseroth

    Has Service

    AAV8

    Insert

    DREADD Gi-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-sgRNA(MS2)

    Plasmid

    #102560

    Purpose

    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET14-GFP15_Encap

    Plasmid

    #86407

    Purpose

    Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

    Depositor

    David Savage

    Article

    Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

    Inserts

    sfGFP with a 15aa CLP tag
    Encapsulin
    

    Use

    Tags

    6x Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCerulean-VSVG

    Plasmid

    #11913

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…

    Promoter

    Available since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-SET-FLAG-HA

    Plasmid

    #24998

    Depositor

    Judy Lieberman

    Article

    Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

    Insert

    SET (SET Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCytERM-mhYFP

    Plasmid

    #186530

    Purpose

    Visualization of the ER in cells using monomeric hyperfolder YFP (compatible with OSER assay)

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Use

    Tags

    Cytochrome p450 first 29 animo acids (Oryctolagus…

    Expression

    Mammalian

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTYB1-T4uvsX

    Plasmid

    #228213

    Purpose

    Expression in BL21(DE3) of T4 recombinase

    Depositor

    Stephen Benkovic

    Insert

    uvsX (uvsX Escherichia phage T4)
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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