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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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179,960 results

Showing: 11301 - 11320 of 179960 results

  6. pEF_dCas9

    Plasmid
    #68416
    Purpose
    Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    68416-plasmid-map-sequence-id-120194

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  7. GCGR-Tango

    Plasmid
    #66291
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GCGR (GCGR Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles

  10. Beta-2-adrenergic receptor-YFP

    Plasmid
    #67146
    Purpose
    Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Beta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)
    Tags
    YFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    67146-plasmid-map-sequence-id-121580

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  11. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Catherine Berlot
    Publication
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    66080-plasmid-map-sequence-id-436984

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  13. pcDNA3-TORCAR-NLS

    Plasmid
    #64931
    Purpose
    Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TORCAR-NLS (EIF4EBP1 Synthetic, Human)
    Tags
    Cerulean, Nuclear localization signal (NLS), and …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64931-plasmid-map-sequence-id-109697

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  15. pHLSec2-FN-YPet

    Plasmid
    #65421
    Purpose
    Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7
    Depositor
    Harold Erickson
    Publication
    Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
    Tags
    YPet
    Expression
    Mammalian
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    65421-plasmid-map-sequence-id-115435

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  16. Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack

    Plasmid kit
    #1000000062
    Purpose
    Plasmids to create all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs for genome engineering using dCas9 and FokI-dCas9. The Multiplex CRISPR/Cas9 Assembly System Kit (kit #1000000055) is required for the use of this accessory pack.
    Depositor
    Takashi Yamamoto
    Application
    Genome Editing
    Vector type
    Mammalian Expression
    Editing type
    CRISPR
    Available since
    June 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. GFP-ATAD2

    Plasmid
    #65370
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATAD2 (ATAD2 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65370-plasmid-map-sequence-id-177053

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  20. pSBtet-GB

    Plasmid
    #60504
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and blasticidin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    60504-plasmid-map-sequence-id-97543

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Showing: 11301 - 11320 of 179960 results