163,421 results

1. pFlagCMV2-EFP

   Plasmid

   #12449

   Depositor

   Dong-Er Zhang

   Article

   Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

   Insert

   EFP (TRIM25 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2333_Cas9-CtIP-dnRNF168

   Plasmid

   #200254

   Purpose

   This plasmid expresses the Cas9-CtIP-dnRNF168 in mammalian cells

   Depositor

   Claudio Mussolino

   Article

   Carusillo et al Nucleic Acids Res. 2023 May 22;51(9):466

   Insert

   Cas9-CtIP-dnRNF168
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV2_HLF-ZE

   Plasmid

   #175034

   Purpose

   HLF targeting construct introducing an ires ZsG P2A tEGFR cassette downstream of the human HLF stop codon

   Depositor

   Guy Sauvageau

   Article

   Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

   Insert

   HLF ires ZsGreen P2A tEGFR
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET11b-SpyCatcher-HA2

   Plasmid

   #165991

   Purpose

   SpyCatcher-fused influenza HA ministem with removable HisTag

   Depositor

   Vesa Hytönen

   Article

   Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

   Insert

   SpyCatcher-HA2
   

   Use

   Tags

   Foldon, HisTag, and SpyCatcher

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME minprom_creERT2

   Plasmid

   #195966

   Purpose

   Gateway pME CreERT2 ORF

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mouse beta-globin minimal promoter creERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

   Plasmid

   #174893

   Purpose

   amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Insert

   mCherry and EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGM9121

   Plasmid

   #51833

   Purpose

   level 0 Universal acceptor

   Depositor

   Sylvestre Marillonnet

   Article

   Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 7, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCMV R-CEPIA3mt

   Plasmid

   #140464

   Purpose

   Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

   Depositor

   Masamitsu Iino

   Article

   Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

   Insert

   R-CEPIA3mt
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2

   Plasmid

   #171104

   Purpose

   Expresses sgRNA (ENO1 gRNA) in Candida auris.

   Depositor

   Teresa O'Meara

   Article

   Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-GfaABC1D-GRAB_g5-HT3.0

    Plasmid

    #208727

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3xFLAG CMV14 / BRAF

    Plasmid

    #131710

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6_B2M_sgRNA(PP7)

    Plasmid

    #232438

    Purpose

    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSEED

    Plasmid

    #219658

    Purpose

    Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Empty SEED Cassette (3xP3-dsRed)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-DeltaFosB

    Plasmid

    #68545

    Purpose

    AAV expression of DeltaFosB

    Depositor

    Eric Nestler

    Article

    Zachariou et al Nat Neurosci. 2006 Feb;9(2):205-11. Epub

    Insert

    DeltaFosB-IRES-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    truncated splice variant of FosB

    Promoter

    CMV

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pLentiRNAGuide_003

    Plasmid

    #192505

    Purpose

    For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-pcDNA3-PKCgamma-cys1Acys1B

    Plasmid

    #21190

    Depositor

    Tobias Meyer

    Article

    Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

    Insert

    PKCgamma-cys1Acys1B (Prkcg Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…

    Promoter

    Available since

    July 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-CAG-jGCaMP8f-WPRE (AAV Retrograde)

    Viral Prep

    #179254-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from AAV-CAG-jGCaMP8f-WPRE (#179254). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8f-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    CAG

    Tags

    None

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

18. σ1-mCherry

    Plasmid

    #186578

    Purpose

    Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    AP1S1 (AP1S1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTT3 Flrt2-myc

    Plasmid

    #72192

    Purpose

    Express full-length Flrt2with a C-terminal myc tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Flrt2 (Flrt2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCRISPR-EGFP

    Plasmid

    #75159

    Purpose

    Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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