163,421 results

1. pNS35 (multiAsCas12a piggyBac)

   Plasmid

   #217336

   Purpose

   piggyBac expression of multiAsCas12a

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   CAG

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Wnt3A

   Plasmid

   #35908

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt3A (WNT3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-TPX2

   Plasmid

   #31227

   Depositor

   Patricia Wadsworth

   Article

   Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88

   Insert

   TPX2 (TPX2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. 7TFC

   Plasmid

   #24307

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-FFluc // SV40-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. mNeptune2-C1

   Plasmid

   #54836

   Purpose

   Localization: C1 Cloning Vector, Excitation: 600, Emission: 650

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mNeptune2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pNR220

   Plasmid

   #79541

   Purpose

   Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Inserts

   TP901
   BxbI
   A118
   

   Use

   Tags

   His tag and LAA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. AID* Kit

   Plasmid Kit

   #1000000120

   Purpose

   Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.

   Depositor

   Helle Ulrich

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

8. AAV GFP

   Plasmid

   #49055

   Purpose

   adeno-associated virus (AAV) encoding a green fluorescent protein

   Depositor

   Fred Gage

   Article

   Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-hSyn-Ribo-jGCaMP8s (AAV1)

   Viral Prep

   #167572-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV-hSyn-Ribo-jGCaMP8s (#167572). In addition to the viral particles, you will also receive purified AAV-hSyn-Ribo-jGCaMP8s plasmid DNA. Synapsin-driven neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Promoter

   Syn

   Tags

   None

   Available since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

10. Str-Ii_VSVG-SBP-mCherry

    Plasmid

    #65301

    Purpose

    synchronize trafficking of VSVG from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. OMM-V5-LOV-Turbo

    Plasmid

    #199665

    Purpose

    expresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    AKAP1 transmembrane domain and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIPZ (M)-HT-Cbx2

    Plasmid

    #82510

    Purpose

    Expresses Halotag-Cbx2 fusion proteins in mammalian cells

    Depositor

    Xiaojun Ren

    Insert

    Chromobox Homolog 2 (CBX2 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cherry-LacRep

    Plasmid

    #18985

    Depositor

    Mirek Dundr

    Article

    Dundr et al J Cell Biol. 2007 Dec 17. 179(6):1095-10

    Insert

    LacI
    

    Use

    Tags

    AviTag, SV40 NLS, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EF1-FHC-POLQ

    Plasmid

    #64875

    Purpose

    lentiviral expression of human POLQ

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-SHP2 CS

    Plasmid

    #8382

    Depositor

    Ben Neel

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C459S, mutation of essential catalytic cysteine

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pBUE411

    Plasmid

    #62200

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    OsU3 and Ubi

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7C-HSP90AB1

    Plasmid

    #177659

    Purpose

    Recombinant protein synthesis of human HSP90AB1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSP90AB1 (HSP90AB1 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFGAMS-mEos2

    Plasmid

    #105128

    Purpose

    Expresses human FGAMS-mEos2 fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    French et al Science. 2016 Feb 12;351(6274):733-7. do

    Insert

    PFAS (PFAS Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Minos ITR loxp MCS lox2272

    Plasmid

    #218983

    Purpose

    Plasmid containing ITR sequences compatible with Minos transposase and multiple cloning site

    Depositor

    Amro Hamdoun

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEG BacMam C term 3C eGFP StrepII

    Plasmid

    #160686

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    3C eGFP StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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