163,403 results

1. pF(UG) hSyn SYP-HaloTag

   Plasmid

   #188907

   Purpose

   Expresses synaptic HaloTag

   Depositor

   Edwin Chapman

   Article

   Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

   Insert

   SYP (Syp Mouse)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pL-CRISPR.EFS.tRFP

   Plasmid

   #57819

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPRi(v1)-Blast

   Plasmid

   #170067

   Purpose

   Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   KRAB-dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2A and 2xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAK501

   Plasmid

   #48107

   Purpose

   lacZ promoter-probe plasmid with pVS1 origin of replication and chloramphenicol resistance

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-mCherry-IRES-Cre

   Plasmid

   #55632

   Purpose

   Expresses Cre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Inserts

   mCherry
   Cre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a and Ef1a/IRES

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFlagCMV2-EFP

   Plasmid

   #12449

   Depositor

   Dong-Er Zhang

   Article

   Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

   Insert

   EFP (TRIM25 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lck-mTurquoise2-iLID

   Plasmid

   #176125

   Purpose

   Plasma membrane localized iLID

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   iLID
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMKO.1 puro

   Plasmid

   #8452

   Purpose

   Retroviral vector for shRNA expression.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Type

   Empty backbone

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO-GAlphasL-RLuc8

   Plasmid

   #140981

   Purpose

   Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphasL-RLuc8 (GNAS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMo130-TelR

    Plasmid

    #50799

    Purpose

    Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

    Depositor

    Kim Lee Chua

    Article

    Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

    Insert

    tellurite-resistance cassette
    

    Use

    Suicide vector

    Tags

    Expression

    Mutation

    Promoter

    PCs12

    Available since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19/mChe-Golgi

    Plasmid

    #183169

    Purpose

    Construct for transient expression of Golgi apparatus-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-Golgi
    

    Use

    Tags

    AtRER1B, A. thaliana endoplasmic reticulum retrie…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSMALB-ATF4.12

    Plasmid

    #155033

    Purpose

    Fluorescent reporter for ATF4 translation, Negative Control

    Depositor

    Peter van Galen, John Dick

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    uORF1 start codon mutated

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKM512

    Plasmid

    #140192

    Purpose

    For curing of Orbit-integrated plasmids and associated drug marker

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    zeocin resistance gene
    Bxb1gp47 (directionality factor)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cloned in gp47 directinality factor in place of R…

    Promoter

    EM7 and Ptet

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b_PstS_1

    Plasmid

    #78198

    Purpose

    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A197C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A197C (please see depositors comment below)

    Promoter

    T7

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid

    #177672

    Purpose

    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells

    Depositor

    Karl Deisseroth

    Has Service

    AAV8

    Insert

    DREADD Gi-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKC31

    Plasmid

    #226777

    Purpose

    Expresses Drosophila UNC-104(R562H) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (R562H) (unc-104 Fly)
    

    Use

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    changed Arginine 562 to Histidine (bris mutation)

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-TA-ERP2

    Plasmid

    #80477

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFM689

    Plasmid

    #74635

    Purpose

    alias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Inserts

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEP4-myc-ACE2

    Plasmid

    #141185

    Purpose

    Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-EGFP-N-IFT80

    Plasmid

    #218731

    Purpose

    Expresses C-terminally EGFP-tagged IFT80 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT80 (IFT80 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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