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Synapsin-cyto-iATPSnFR1.0

Plasmid

#102556

Purpose

expresses cytoplasmic ATP sensor in neurons

Depositor

Baljit Khakh

Publication

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

AAV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET21a(+)-Histag-mCherry (527)

Plasmid

#70719

Purpose

Expression vector for mCherry purification

Depositor

Richard Murray

Publication

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

mCherry

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCE046-SiT-Cas12a-[Repr]

Plasmid

#128132

Purpose

Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Publication

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Repr]-Triplex

Use

AAV

Expression

Mammalian

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFC330

Plasmid

#87842

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

Depositor

Uffe Mortensen

Publication

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
pyrG

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and native pro…

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pENN236_CRISPRcharm_Kv2

Plasmid

#220839

Purpose

CRISPRcharm Kv2 expression in mammalian cells

Depositor

Jonathan Weissman

Publication

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm Kv2

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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PX408 Francisella tularensis subsp. novicida Cas9

Plasmid

#68705

Purpose

Francisella tularensis subsp. novicida Cas9

Depositor

Feng Zhang

Insert

FtCas9

Use

CRISPR

Tags

HA-NLS

Expression

Mammalian

Promoter

CMV

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Human ubiquitination-related proteins CRISPR KO library

Pooled library

#174592

Purpose

Targets human ubiquitation-related proteins and deubiquitinating enzymes.

Depositor

Juan Bonifacino

Publication

Jia et al Elife. 2019 Nov 6;8. pii: 50034. doi: 10

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET28a 6xHis PreScission SARS-CoV-2 nsp13

Plasmid

#159390

Purpose

Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

Depositor

Tarun Kapoor

Publication

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression

Tags

His6 tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHR_Gal4UAS_ Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_P2A_antiCTLA4scFv_PGK_mCherry

Plasmid

#85436

Purpose

Lentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expression

Depositor

Wendell Lim

Publication

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Pembrolizumab and anti-CTLA4 scFv

Use

Lentiviral

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSuperRetro control

Plasmid

#11189

Depositor

Bruce Spiegelman

Publication

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Type

Empty backbone

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRES-GFP

Plasmid

#32477

Purpose

Cre-dependent expression of PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Publication

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F-5HT3HC

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Promoter

Synapsin

Available since

Jan. 12, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-TRE-mRuby2

Plasmid

#99114

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-PE6f

Plasmid

#207856

Purpose

Mammalian expression of PE6f prime editor

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6f

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-PE6g

Plasmid

#207857

Purpose

Mammalian expression of PE6g prime editor

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6g

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-MMLVgag-3xNES-ABE7.10-NG

Plasmid

#181753

Purpose

MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Publication

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE7.10-NG

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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His6-SpyCatcher-mi3

Plasmid

#113043

Purpose

Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Publication

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

His6-SpyCatcher-mi3

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJZC43

Plasmid

#66565

Purpose

sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2XPP7
PCP-VP64 IRES mCherry

Expression

Mammalian

Promoter

CMV and U6

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pDW363

Plasmid

#8842

Depositor

David Waugh

Publication

Tsao et al Gene. 1996 Feb 22. 169(1):59-64.

Type

Empty backbone

Tags

MBP and biotin acceptor peptide

Expression

Bacterial

Available since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PET30B-PTEN

Plasmid

#20741

Depositor

Alonzo Ross

Publication

Redfern et al Biochemistry. 2008 Feb 19. 47(7):2162-71

Insert

PTEN (PTEN Human)

Tags

6xHis

Expression

Bacterial

Available since

April 2, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
MuHKII-pGFPN3

Plasmid

#21922

Depositor

Hossein Ardehali

Publication

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Mutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is the full length human HKII cDNA sequence …

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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