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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,317 results for: golden gate

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Showing: 1121 - 1140 of 5317 results

  1. pR2B5

    Plasmid
    #245486
    Purpose
    pL1-R2-pAtUBI-RUBY-tRBCS (Golden Gate Level 1 module with a transcription unit expressing RUBY from the AtUBI promoter with the tRBC terminator for R2 position in Level 2 vector)
    Depositor
    Beth Rowan
    Insert
    pAtUBI-RUBY-tRBCS
    Expression
    Plant
    Promoter
    Polyubiquitin 10 gene from Arabidopsis thaliana
    Available since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    245486-plasmid-map-sequence-id-492313

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  2. pR2B6

    Plasmid
    #245487
    Purpose
    pL1-R2-pLsUBI-RUBY-tLsUBI (Golden Gate Level 1 module with a transcription unit expressing RUBY from the LsUBI promoter with the tLsUBI terminator for R2 position in Level 2 vector)
    Depositor
    Beth Rowan
    Insert
    pLsUBI-RUBY-tLSUBI
    Expression
    Plant
    Promoter
    Polyubiquitin 4 gene (LOC111919935) from lettuce
    Available since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    245487-plasmid-map-sequence-id-492314

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  4. pYL001

    Plasmid
    #249611
    Purpose
    Cloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 with mutation SlFT element
    Depositor
    Steven Jacobsen
    Publication
    Liu et al bioRxiv 2025.11.08.687402
    Type
    Empty backbone
    Expression
    Plant
    Available since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    249611-plasmid-map-sequence-id-496977

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  6. MoChlo: Modular Cloning Chloroplast Toolbox

    Plasmid kit
    #1000000156
    Purpose
    Library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.
    Depositor
    Scott Lenaghan
    Application
    Cloning and Synthetic Biology
    Vector type
    Plant Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only

  7. IBMc233

    Plasmid
    #161939
    Purpose
    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Publication
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    Expression
    Bacterial
    Promoter
    PPhlF
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161939-plasmid-map-sequence-id-327549

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  17. Cultivarium POSSUM Toolkit

    Plasmid kit
    #1000000234
    Purpose
    Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model bacteria.
    Depositor
    Nili Ostrov
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate (Loop)
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only

  18. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Publication
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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Showing: 1121 - 1140 of 5317 results