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pAc5.1B-lambdaN-HA-CeAIN2-W282AW390A_O

Plasmid

#147143

Purpose

Insect Expression of CeAIN2-W282AW390A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W282AW390A

Expression

Insect

Mutation

Deletion of 2 AA SD at position 378-379 compared …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2-W366A_O

Plasmid

#147144

Purpose

Insect Expression of CeAIN2-W366A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W366A

Expression

Insect

Mutation

Deletion of 2 AA SD at position 378-379 compared …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2-W366AW390A_O

Plasmid

#147145

Purpose

Insect Expression of CeAIN2-W366AW390A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W366AW390A

Expression

Insect

Mutation

Deletion of 2 AA SD at position 378-379 compared …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2-W390A_O

Plasmid

#147146

Purpose

Insect Expression of CeAIN2-W390A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W390A

Expression

Insect

Mutation

Deletion of 2 AA SD at position 378-379 compared …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2-3xWmut_O

Plasmid

#147139

Purpose

Insect Expression of CeAIN2-3xWmut

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-3xWmut

Expression

Insect

Mutation

Deletion of 2 AA SD at position 378-379 compared …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2-W282A_O

Plasmid

#147141

Purpose

Insect Expression of CeAIN2-W282A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W282A

Expression

Insect

Mutation

Deletion of 2 AA SD at position 378-379 compared …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-CeAIN2-W282AW366A_O

Plasmid

#147142

Purpose

Insect Expression of CeAIN2-W282AW366A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W282AW366A

Expression

Insect

Mutation

Deletion of 2 AA SD at position 378-379 compared …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2_254-400-V5His6_R

Plasmid

#147407

Purpose

Insect Expression of CeAIN2_254-400

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-RanGAP*

Plasmid

#175237

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the Km

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-Gal4

Plasmid

#62899

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCPP5233

Plasmid

#128715

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

Insert

shcM-hopM1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5951

Plasmid

#128719

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

Insert

hopR1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4

Plasmid

#62900

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-Gal4

Plasmid

#62901

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3flag-RanGAPdeltaN

Plasmid

#25104

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

RanGAP1 deleted N-term domain (RANGAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deleted amino acids 1-203.

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pET28a-SdrG-B2-ELP-HIS-ybbR

Plasmid

#169134

Purpose

E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Article

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G

Tags

3xELP linker, 6x Histag, and ybbr tag

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG-B1-ELP-HIS-ybbR

Plasmid

#169133

Purpose

E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Article

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G

Tags

3xELP linker, 6x Histag, and ybbr tag

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690_R

Plasmid

#147393

Purpose

Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-HsTNRC6C_1207-1690 (TNRC6C Human, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-RanGAP*

Plasmid

#175238

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG-FLN-ELP-His-ybbr

Plasmid

#168047

Purpose

Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G

Tags

3xELP linker, 6x Histag, ddFLN4, and ybbr tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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