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pCAG-MBP-ULK1-290-835-mcherry

Plasmid

#249733

Purpose

Expressing human ULK1-290-835 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Publication

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

290-835

Promoter

CAG

Available since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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MultiCAST-miniTn-Carb

Plasmid

#248975

Purpose

MultiCAST mini-Tn donor plasmid containing ampicillin/carbenicillin selectable marker

Depositor

Matthew Waldor

Publication

Basta et al bioRxiv [Preprint]. 2025 Nov 1:2025.10.3

Type

Empty backbone

Expression

Bacterial

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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dSpCas9-SmBiT

Plasmid

#236759

Purpose

Split NanoLuc luciferase biosensor fused to dSpCas9

Depositor

David Segal

Publication

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

NanoLuc SmBit

Tags

dSpCas9

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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dSaCas9-NLuc

Plasmid

#238947

Purpose

C-terminal fusion of NanoLuc luciferase to dSaCas9, mammalian expression

Depositor

David Segal

Publication

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

NanoLuc

Tags

dSaCas9

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA4-MS2-CP-GFP-SBP

Plasmid

#249048

Purpose

Expresses MS2 cap binding protein (CP) fused to GFP and streptavidin binding peptide (SBP)

Depositor

Jeffrey Gerst

Publication

Slobodin et al RNA. 2010 Nov;16(11):2277-90. doi: 10.12

Insert

MS2 binding coat protein

Tags

GFP and streptavidin binding peptide

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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TOMM70-FKBP12-Halo donor plasmid

Plasmid

#239867

Purpose

endogenously tags TOMM70 with Halo tag

Depositor

Derek Narendra

Publication

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TOMM70 (TOMM70 Human)

Use

Donor plasmid

Tags

Halo Tag

Available since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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EML8552_pEML41003-tufApst4-15

Plasmid

#237865

Purpose

A suicide vector for replacing 4th to 15th amino acids of tufA

Depositor

Erh-Min Lai

Publication

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

tufA

Available since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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EML8553_ pEML41003-tufBpst4-15

Plasmid

#237866

Purpose

A suicide vector for replacing 4th to 15th amino acids of tufB

Depositor

Erh-Min Lai

Publication

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

tufB

Available since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGRNA2II_LacZT1

Plasmid

#247079

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets Ecoli LacZ T1 site

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Venus

Plasmid

#247761

Purpose

mammalian expression of Venus

Depositor

Alan Ashworth

Publication

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

Venus

Expression

Mammalian

Available since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ECFP

Plasmid

#247760

Purpose

mammalian expression of ECFP

Depositor

Alan Ashworth

Publication

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

ECFP

Expression

Mammalian

Available since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX_Venus

Plasmid

#247758

Purpose

Lentiviral expression of Venus in mammalian cells

Depositor

Alan Ashworth

Publication

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

Venus

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGRNA3_LacZ_B1_IIB5

Plasmid

#247081

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets both Ecoli LacZ B1 and B5 site

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pBBR-sfGFP N149TAG-MjtRNA

Plasmid

#242001

Purpose

Expresses MjtRNA and contains sfGFP gene with amber codon at 149 position.

Depositor

Wei Niu

Publication

He et al ACS Synth Biol. 2022 Nov 18;11(11):3724-

Insert

sfGFPN149TAG

Expression

Bacterial

Available since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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Venus-giantin

Plasmid

#234749

Purpose

Mammalian expression of Golgi marker Venus-giantin

Depositor

Nevin Lambert

Publication

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Venus-giantin

Expression

Mammalian

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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MCP/PCP yellow RNA Lantern

Plasmid

#226096

Purpose

RNA tracking

Depositor

Jennifer Prescher

Publication

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-Venus d12-LgBiT-FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcRII-mogat3a in situ probe

Plasmid

#232135

Purpose

zebrafish mogat3a anti-sense in situ probe, linearize with NotI, SP6 polymerase

Depositor

Steven Farber

Publication

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat3a

Use

In vitro transcription

Promoter

SP6/T7

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p140Tol2-Olactb:SABER

Plasmid

#226829

Purpose

Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.

Depositor

Bushra Raj

Publication

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

BFP-SABER
mCherry-t2A-CreERT2

Use

Zebrafish expression

Promoter

Olactb and hsp70

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-SAV

Plasmid

#191200

Purpose

Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & Streptavidin fusion

Tags

6xHis and Streptavidin core domain, amino acids 1…

Expression

Bacterial

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2699-CMV-Nme2Cas9-E932R

Plasmid

#228720

Purpose

Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Publication

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nme2Cas9-E932R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 E932R

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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