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pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG

Plasmid

#130684

Purpose

Expresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D453_F489del, E653_G1960del

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG

Plasmid

#130683

Purpose

Expresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D409_E452del, E653_G1960del

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw CBX8 quad

Plasmid

#121118

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 (R19A,R20A, R22A, W32A) (CBX8 Human)

Use

Lentiviral

Tags

V5

Mutation

R19A, R20A, R22A, W32A

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-MAPKAP1RBD

Plasmid

#120710

Purpose

Expresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MAPKAP1 RBD

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Amino acids 279-353

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-MORC2 ts3

Plasmid

#115888

Purpose

MORC2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

MORC2 shRNA (MORC2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SUV39h2 ts3

Plasmid

#115885

Purpose

SUV39h2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h2 shRNA (SUV39H2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SUV39h2 ts2

Plasmid

#115884

Purpose

SUV39h2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h2 shRNA (SUV39H2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SUV39h2 ts1

Plasmid

#115883

Purpose

SUV39h2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h2 shRNA (SUV39H2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SUV39h1 ts3

Plasmid

#115882

Purpose

SUV39h1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h1 shRNA (SUV39H1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-8A/S15D

Plasmid

#114031

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 8A/S15D

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pX459V2.0+N497A+Q695A+Q926A

Plasmid

#108300

Purpose

pX459 V2.0 (Plasmid #62988) with the N497A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR V2 sgMTHFD1L_2

Plasmid

#106317

Purpose

Express Cas9 and sgRNA targeting MTHFD1L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD1L

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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p7142 pHAGE-P-CMVt-N-HA-GAW-ZFYV9

Plasmid

#100162

Purpose

Expresses ZFYV9

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

ZFYV9 (ZFYVE9 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgC17orf89-2

Plasmid

#86135

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting C17orf89 (NDUFAF8 )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-3xFLAG-OB D166R

Plasmid

#79061

Purpose

Retroviral expression of 3xFLAG tagged, nuclear localized TPP1-OB-RR folding domain

Depositor

Steven Artandi

Article

Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

Insert

TPP1 (TPP1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Aspartic acid 166 changed to Arginine

Promoter

CMV

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only
pREG(R1-R4) #BGV059

Plasmid

#48991

Purpose

Retroviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Retroviral

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pLKO-sh-hSH3BP4

Plasmid

#46895

Purpose

expression of shRNA against human SH3BP4

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (SH3BP4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

W92A (mutated SH3 domain)

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pLKO-sh-mSH3BP4

Plasmid

#46894

Purpose

expression of shRNA against mouse SH3BP4

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (Sh3bp4 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

W92A (mutated SH3 domain)

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pLKO.1 - TRC cloning vector

Plasmid

#10878

Purpose

3rd generation transfer plasmid

Depositor

David Root

Article

Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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CROPseq-iT7

Plasmid

#211699

Purpose

NIS-Seq-compatible lentiviral vector for expressing sgRNAs

Depositor

Jonathan Schmid-Burgk

Article

Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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