We narrowed to 4,785 results for: Drosophila
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Plasmid#17767DepositorInsertdme-miR-1003 minigene
UseDrosophila p-element vectorAvailable SinceMay 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmImp8_G
Plasmid#146365PurposeInsect Expression of DmImp8DepositorInsertDmImp8
ExpressionInsectAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-CG3475_A
Plasmid#145858PurposeInsect Expression of CG3475-3UTRDepositorInsertCG3475-3UTR
ExpressionInsectAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-Act5c
Plasmid#130278PurposeDrosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.DepositorAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔN
Plasmid#182840PurposeA 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
TagsFLAG tagExpressionInsectPromoterMTAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHD-dsRed-attP_dCrk_flankingHRs
Plasmid#131141PurposeContains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).DepositorUseCRISPR and Cre/Lox; Homology directed repair temp…Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-Hsp70-Cas9
Plasmid#46294PurposeA codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.DepositorInsertcodon optimized Cas9
UseCRISPRTags3x FlagExpressionInsectMutationSilent change from C to A at bp 834 of insertPromoterHsp70Available SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pB[vasa-Cas9 PUb-ZsGreen]
Plasmid#169012PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacExpressionInsectPromoterDrosophila melanogaster vasaAvailable SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Renilla luciferase-Pol III
Plasmid#37380DepositorInsertPolIII-Renilla control reporter (RpIII128 Fly)
UseLuciferaseExpressionMammalianPromoterRNA PolIII 128 subunit (RpIII128)Available SinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-AlstR-IRES2-EGFP
Plasmid#14895DepositorAvailable SinceMay 11, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBac{3XP3::DsRed, Pactin::Ptrsps}
Plasmid#86862PurposeExpresses DsRed in eyes and ubiquitous expression of P-transposaseDepositorInsertpACNNTNPII-S129A
ExpressionInsectMutationS129AAvailable SinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D CA
Plasmid#52247PurposeDrosophila expression of YFP-Ras85D constitutively active mutantDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ61L; CA mutationPromoterGAL4Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pB[nos-Cas9 PUb-ZsGreen]
Plasmid#169011PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacExpressionInsectPromoterDrosophila melanogaster nanosAvailable SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBac{3XP3::EGFP, Pactin::Ptrsps}
Plasmid#86861PurposeExpresses EGFP in eyes and ubiquitous expression of P-transposaseDepositorInsertpACNNTNPII-S129A
ExpressionInsectAvailable SinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab5 WT
Plasmid#53468DepositorAvailable SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D WT
Plasmid#52242PurposeDrosophila expression of YFP-Ras85DDepositorAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGC(2.1)-U6.3
Plasmid#170516PurposePCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFPDepositorInsertgRNAcore(2.1)-pU6.3
UsePcr template vector for amplifying grnacore(2.1)-…Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras85D DN
Plasmid#52246PurposeDrosophila expression of YFP-Ras85D dominant negative mutantDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS17N; DN mutationPromoterGAL4Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-2_dCrk
Plasmid#131146PurposeExpresses GST::dCrk (based on crk isoform RA).DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -