We narrowed to 18,204 results for: puro

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BRCA1 A10.2 gRNA

Plasmid

#90549

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA1 (Guide Designation A10.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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BRCA2 A11.2 gRNA

Plasmid

#90550

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA2 (Guide Designation A11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-Pur-CAG-hM4Di-mCherry

Plasmid

#80947

Purpose

human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

Insert

hM4Di-mCherry

Tags

mcherry fusion

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pRetroX GFP T2A Cre

Plasmid

#63704

Purpose

Inducible expression of GFP T2A Cre fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

T2A Cre

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-HsSAS6

Plasmid

#136789

Purpose

Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-SAS6 (SASS6 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-Tet-OsTIR1(WT)-V5

Plasmid

#158663

Purpose

Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

Tet-OsTIR1(WT) AAVS1

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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H52-pUCM-hNGN2-inv

Plasmid

#110492

Purpose

Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

Depositor

Michael Ward

Inserts

Neurogenin 2 (NEUROG2 Human)
mcherry
rtTA3G

Use

CRISPR, Cre/Lox, and TALEN

Expression

Mammalian

Promoter

CAG, EF-1 alpha, and TRE3G

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSCALPS-FLAG-HA-mmuMettl3

Plasmid

#207122

Purpose

Lentiviral plasmid to express mouse Mettl3

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Mettl3 (Mettl3 Mouse)

Use

Lentiviral

Tags

FLAG-HA

Promoter

SSFV

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEAK-Flag/INPP4B

Plasmid

#24324

Depositor

Lewis Cantley

Article

Gewinner et al Cancer Cell. 2009 Aug 4. 16(2):115-25.

Insert

Inositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

none

Available Since

May 11, 2010

Availability

Academic Institutions and Nonprofits only

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p6622 MSCV-P C HA GLTSCR

Plasmid

#34902

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

GLTSCR1 (BICRA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-metap2

Plasmid

#100510

Purpose

Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

METAP2 (METAP2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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p23-NES-EsCas13d-msfGFP-NES-Flag

Plasmid

#165074

Purpose

overexpression of EsCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

EsCas13d

Expression

Mammalian

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLCRISPR-CMV-cGAS3

Plasmid

#102610

Purpose

CRISPR/Cas9 lentivector targeting human cGAS

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

gRNA targeting human cGAS

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMTL3

Plasmid

#127966

Purpose

constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

AAVS1-CAG-KRAB-dCas9-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDM002-sgRNA-lenti-ZeoR

Plasmid

#200060

Purpose

Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

Bleomycin resistance, mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-mCasp8

Plasmid

#208782

Purpose

Inducibly expresses mouse caspase8 fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

mCherry mCaspase8 Cry2 (Casp8 Mouse)

Use

Lentiviral

Tags

Cry2 and mCherry

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLX317-VRK1

Plasmid

#199643

Purpose

Expresses VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK1 (VRK1 Human)

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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FOXM1 C11.2 gRNA

Plasmid

#90690

Purpose

3rd generation lentiviral gRNA plasmid targeting human FOXM1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FOXM1 (Guide Designation C11.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 NT sgRNA3

Plasmid

#164929

Purpose

Expresses Non-targeting sgRNA3 control in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

Non-targeting sgRNA3 (verified for knockout)

Use

Lentiviral

Promoter

EFS promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCW-OVOL2-HA

Plasmid

#184737

Purpose

Doxycycline-inducible lentiviral expression of OVOL2

Depositor

Alessia Ciarrocchi, Gloria Manzotti

Article

Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

Insert

ovo like zinc finger 2 (OVOL2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

Tet ON

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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