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5mARF17 pGreenII0129

Plasmid

#12081

Depositor

David Bartel

Article

Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

Insert

5mARF17 (ARF17 Mustard Weed)

Use

Plant transformation via agrobacterium

Expression

Plant

Mutation

miR160-resistant version of ARF17 has five silent…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MFF iso5 S155A

Plasmid

#74381

Purpose

Flag tagged human S155A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Ala (numbering based on MFF isoform 1)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pCIG AKT3 E17K;K177M

Plasmid

#73049

Purpose

Human AKT3 E17K;K177M

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 E17K;K177M (AKT3 Human)

Expression

Mammalian

Mutation

E17K;K177M

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.6-Fc-His

Plasmid

#72103

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 6 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.6 (Nrp2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.2-Fc-His

Plasmid

#72099

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.2 (Nrp2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.30 EC10-Fc-His

Plasmid

#72190

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.30

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp2.3-AP-His

Plasmid

#71974

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.3 (Nrp2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Tet1d834-1053-Poly(A)

Plasmid

#65561

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1d834-1053 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

del(834-1053)

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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M26I DJ-1 TEV site pET15b

Plasmid

#60685

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26I DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26I and TEV site was inserted (see comments belo…

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
M26L DJ-1 TEV site pET15b

Plasmid

#60686

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26L DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26L and TEV site was inserted (see comments belo…

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA4 rat NZAP

Plasmid

#45908

Depositor

Harmit Malik

Article

Kerns et al PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1

Insert

Zc3hav1 zinc finger CCCH type, antiviral 1 (Zc3hav1 Rat)

Tags

HA

Expression

Mammalian

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only
EGFP-hPMCA4b delta6

Plasmid

#47590

Depositor

Emanuel Strehler

Article

Padanyi et al Biochim Biophys Acta. 2009 Jun;1793(6):1

Insert

PMCA4bdelta6 (ATP2B4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

6 C-terminal AA deleted (needed for PDZ binding)

Promoter

CMV

Available Since

Aug. 26, 2013

Availability

Academic Institutions and Nonprofits only
pEBB-3XMyc-TRAF2 S274A

Plasmid

#44107

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S274A (TRAF2 Human)

Tags

3xMyc

Expression

Mammalian

Mutation

Serine 274 to Alanine

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
GoldyTALEN-ppp1cab TAL1

Plasmid

#41835

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

protein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)

Use

Cloning vector

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 Slp2-a C2AB

Plasmid

#40058

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a C2AB (Sytl2 Mouse)

Tags

GST

Expression

Bacterial

Mutation

C2AB domains only

Promoter

Tac

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
EYFP-Abr NE/AA

Plasmid

#36419

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

active BCR-related (ABR Human)

Tags

EYFP

Expression

Mammalian

Mutation

N245A, E246A

Available Since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
K349CLM-6His 215/347

Plasmid

#24451

Depositor

Ron Vale

Article

Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

Insert

Kinesin (KIF5B Human)

Tags

His

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S, E215C, E347C

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
K222C/E334C

Plasmid

#24435

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C/E…

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
A337C/Y344C

Plasmid

#24434

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C/Y…

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
CLM RP KGF Mut 1

Plasmid

#24166

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only