We narrowed to 15,989 results for: LEA

1. pcDNA-NES-ZapCmR1.1

   Plasmid

   #59014

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
   

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pGBW-m4046832

   Plasmid

   #145691

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLIC.B3.TM0851

   Plasmid

   #11530

   Depositor

   Sung-Hou Kim

   Article

   Liu et al J Mol Biol. 2005 Jul 29. 350(5):987-96.

   Insert

   heat-inducible transcriptional repressor (TM0851 T. maritima)
   

   Tags

   HisTag, TEV cleavable

   Expression

   Bacterial

   Available Since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-REPORT(EF1a no GFP) mnuctdTomato

   Plasmid

   #172342

   Purpose

   mnuctdTomato

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4046907

   Plasmid

   #149262

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 nsp2

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;Myc

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   May 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGBW-m4046953

   Plasmid

   #145764

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF7b

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLI_BFP_C-tag TNF

   Plasmid

   #171187

   Purpose

   inducible expression of C-tag TNF

   Depositor

   Veit Hornung

   Article

   Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

   Insert

   C-tag TNF
   

   Use

   Lentiviral

   Tags

   C-tag (internal)

   Expression

   Mammalian

   Mutation

   ADAM cleavage site of TNF (AA73-76) was exchanged…

   Promoter

   tight TRE promoter

   Available Since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBW-m4046891

   Plasmid

   #145749

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF7a protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA3 Glt1a

   Plasmid

   #98705

   Purpose

   mammalian expression of Glt1a

   Depositor

   Paul Rosenberg

   Article

   Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

   Insert

   Glt1a (Slc1a2 Rat)
   

   Expression

   Mammalian

   Mutation

   I288V (please see depositor comments below)

   Promoter

   CMV

   Available Since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBW-m4136454

    Plasmid

    #149529

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    May 13, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pET21a - CPX

    Plasmid

    #89620

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Cytosolic Tryparedoxin peroxidase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO_Lyn11-STf

    Plasmid

    #182008

    Purpose

    CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    Lyn11-STf
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBW-m4046919

    Plasmid

    #145615

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGBW-m4046890

    Plasmid

    #145740

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. D. vulgaris Cas7/pHMGWA

    Plasmid

    #81184

    Purpose

    Expresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas7
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBW-m4095420

    Plasmid

    #149523

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    d1-745 d1063-1945

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGBW-m4046949

    Plasmid

    #145604

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCAG-Trex2-bpA

    Plasmid

    #86984

    Purpose

    Expression of mouse Trex2

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Insert

    mouse Trex2 coding region (Trex2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Plas-CRISPR_reporter

    Plasmid

    #157658

    Purpose

    CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

    Depositor

    Frank Albert

    Article

    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

    Insert

    dCAS9-VP64, mCherry, KanMX
    

    Use

    Insert storage (replicative in e. coli)

    Mutation

    N28D in mCherry- please see depositor comments be…

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIII

    Plasmid

    #80864

    Purpose

    Phagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    M13 gene III
    cI4A5C6G7G,P
    

    Expression

    Bacterial

    Mutation

    238G to 238D (please see depositor comment below)

    Promoter

    BBa_J23106 and rpoN

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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