Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,048 results for: Hal

Showing: 11721 - 11740 of 18048 results

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p-mCherry-enCas9(D10A)

Plasmid

#249063

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9-SRDX (M30)

Plasmid

#239484

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-SRDX

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9-BFP (M39)

Plasmid

#239485

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9-cSRDX (M40)

Plasmid

#239486

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-XTEN16-SRDX

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TET1cd-dCas9-p300 (D18)

Plasmid

#239489

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-p300

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TET1cd-dCas9-IBM1cd (D24)

Plasmid

#239490

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-IBM1cd

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TET1cd-dCas9-IBM1 (D30)

Plasmid

#239492

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-IBM1

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MQ1v-dCas9-KRAB (M22)

Plasmid

#239483

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-MQ1v-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-KRAB

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-ColH-HylB

Plasmid

#248631

Purpose

expresses Myc-tagged ColH and His-tagged HylB as two separate proteins in E. coli

Depositor

Edwin Manuel

Article

Avsharian et al Biomolecules. 2024 Nov 17;14(11):1458. d

Insert

Myc-tagged bacterial collagenase, ColH, and His-tagged hyaluronidase, HylB

Tags

His and Myc

Expression

Bacterial

Promoter

araBAD

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-D166Y

Plasmid

#248123

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with D166Y mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-D166Y (SNAP25 Human)

Expression

Mammalian

Mutation

D166Y

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-I192N

Plasmid

#248126

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with I192N mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-I192N (SNAP25 Human)

Expression

Mammalian

Mutation

I192N

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-K40E

Plasmid

#248127

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with K40E mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-K40E (SNAP25 Human)

Expression

Mammalian

Mutation

K40E

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-WT

Plasmid

#248121

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptosomal-Associated Protein (SNAP-25)

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B (SNAP25 Human)

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-R59P

Plasmid

#248129

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with R59P mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-R59P (SNAP25 Human)

Expression

Mammalian

Mutation

R59P

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-L50S

Plasmid

#248128

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with L50S mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-L50S (SNAP25 Human)

Expression

Mammalian

Mutation

L50S

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-A199G

Plasmid

#248122

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with A1999G mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-A199G (SNAP25 Human)

Expression

Mammalian

Mutation

A199G

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-G43R

Plasmid

#248124

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with G43R mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-G43R (SNAP25 Human)

Expression

Mammalian

Mutation

G43R

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-V48F

Plasmid

#248130

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with V48F mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-V48F (SNAP25 Human)

Expression

Mammalian

Mutation

V48F

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-I67N

Plasmid

#248125

Purpose

Mammalian expression lentiviral vector encoding the Synaptosomal-Associated Protein (SNAP-25) with I67N mutation

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B-I67N (SNAP25 Human)

Expression

Mammalian

Mutation

I67N

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-9 (nonfunctional)

Plasmid

#246292

Purpose

Evaluation of AtU6.29c13 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

AtU6.29c13 promoter (nonfunctional)

Use

CRISPR

Expression

Plant

Mutation

deletions: -A (-58 from TSS), -T (-57) within USE

Promoter

AtU6.29c13 promoter (nonfunctional)

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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Showing: 11721 - 11740 of 18048 results

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