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We narrowed to 62,357 results for: cloning

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Showing: 11761 - 11780 of 62357 results

  1. pENTR_AtU6p_BsaI_gRNA

    Plasmid
    #225987
    Purpose
    To make Gateway entry clone of CRISPR guide RNA under AtU6p
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225987-plasmid-map-sequence-id-448010

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  2. Hs.HRAS Q61K

    Plasmid
    #83187
    Purpose
    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61K mutation
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61K
    Available Since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83187-plasmid-map-sequence-id-158150

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  3. Hs.KRAS4a Q61R

    Plasmid
    #83172
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61R
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83172-plasmid-map-sequence-id-158135

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  4. Hs.KRAS4b Q61R

    Plasmid
    #83135
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61R
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83135-plasmid-map-sequence-id-158098

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  9. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid
    #164637
    Purpose
    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2
    Depositor
    Günter Schneider
    Insert
    target guide sequence PRMT5 inhibition (PRMT5 Human)
    Use
    CRISPR and Lentiviral; Interference
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164637-plasmid-map-sequence-id-326103

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  11. p3015b

    Plasmid
    #209317
    Purpose
    Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.
    Depositor
    Thomas Hooven
    Article
    Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1
    Insert
    Group B Streptococcus-compatible single guide RNA
    Expression
    Bacterial
    Promoter
    Xyl/tet promoter that is constitutively active
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    209317-plasmid-map-sequence-id-435309

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  12. pMDC32B-BS-AtMIR390a-B/c

    Plasmid
    #199560
    Purpose
    Plant expression vector (2x35S) for direct cloning of amiRNAs into Arabidopsis thaliana MIR390a precursor including only the basal stem
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Nucleic Acids Res. 2023 Oct 27;51(19):10
    Insert
    Basal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)
    Use
    RNAi
    Promoter
    2x35S
    Available Since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    199560-plasmid-map-sequence-id-398170

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  13. HBT_pcoCASphi_version1

    Plasmid
    #197931
    Purpose
    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Insert
    pcoCasphi-2
    Use
    CRISPR
    Tags
    FLAG tag and SV40 NLS and nucleoplasmin NLS
    Expression
    Plant
    Promoter
    35SPPDK promoter
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197931-plasmid-map-sequence-id-388007

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  14. P[acman]-Alpha2

    Plasmid
    #165859
    Purpose
    Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165859-plasmid-map-sequence-id-336455

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Showing: 11761 - 11780 of 62357 results