We narrowed to 18,204 results for: puro

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FLAG-TKFC in pLJM60

Plasmid

#154206

Purpose

Lentiviral vector for stable expression of TKFC

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

TKFC (DAK Human)

Use

Lentiviral

Tags

N terminal FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLX317-VRK2

Plasmid

#199641

Purpose

Expresses VRK2

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK2 (VRK2 Human)

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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3xFlag-HaloTag-SUZ12 HDR - pDY082

Plasmid

#171110

Purpose

Homology directed repair plasmid for generating HaloTag-SUZ12 cells

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

SUZ12 (SUZ12 Human)

Tags

HaloTag

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-mCherry-Vav1

Plasmid

#50044

Purpose

Expression of mCherry-Vav1 in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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stabilized HP35 tension sensor module

Plasmid

#101251

Purpose

Expresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.

Depositor

Carsten Grashoff

Article

Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

Insert

YPet(short)-HP35st-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMRXIP Lamp1-Venus

Plasmid

#89937

Purpose

Expresses Lamp1 tagged with Venus in mammalian cells

Depositor

Noboru Mizushima

Article

Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

Insert

rat Lamp1 (Lamp1 Rat)

Use

Retroviral

Tags

Venus

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1 sgTollip

Plasmid

#196546

Purpose

Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pJP-CoV2leader-nLuc-TSS

Plasmid

#175430

Purpose

Expresses nLuc with 5' leader of CoV2 in mammalian cells.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nLUC plus 5' CoV2 leader

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLX317-hcRED

Plasmid

#115440

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

hcRED

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTargeting_Mettl3C_FKBP-V

Plasmid

#165421

Purpose

Template for knockin FBKP-V into Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

Mettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)

Use

Mouse Targeting

Tags

FKBP-V

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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px459-Hira KO sgRNA

Plasmid

#186938

Purpose

Hira KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Hira KO sgRNA (Hira Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS2

Plasmid

#127127

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJP-HBB-nLuc

Plasmid

#175431

Purpose

Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nLUC plus 5' HBB leader

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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shTBX5 # 2

Plasmid

#42550

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTBX5 (TBX5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pLKO.shHmga2

Plasmid

#32399

Depositor

Tyler Jacks, Monte Winslow

Article

Winslow et al Nature. 2011 May 5. 473(7345):101-4.

Insert

shRNA targetting Hmga2 (Hmga2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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shTBX5 # 1

Plasmid

#42549

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTBX5 (TBX5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2 EGFP sgRNA2

Plasmid

#164933

Purpose

Expresses EGFP sgRNA2 in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

sgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)

Use

Lentiviral

Promoter

EFS promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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p23-NES-AdmCas13d-msfGFP-NES-Flag

Plasmid

#165075

Purpose

overexpression of AdmCas13d in human cells

Depositor

Ling-Ling Chen

Article

Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

Insert

AdmCas13d

Expression

Mammalian

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-PmNG

Plasmid

#232201

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pENN456_TALEcharm_Kv2_MmT8x

Plasmid

#220835

Purpose

TALEcharm expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

TALEcharm Kv2

Use

Lentiviral and TALEN

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EF1a

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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