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Showing: 2901 - 2950 of 4641 results

  3. pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197063
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197063-plasmid-map-sequence-id-408409

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  7. FLINC-AKAR1(TA)-CAAX

    Plasmid
    #87706
    Purpose
    Negative-control mutant of FLINC-AKAR1-CAAX.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    FLINC-AKAR1(TA)-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87706-plasmid-map-sequence-id-171088

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  8. PKAsub-TagRFPT-CAAX

    Plasmid
    #87712
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub-TagRFPT-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87712-plasmid-map-sequence-id-176157

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  10. pSB65 - pL0_tGFP-I (CDS1)

    Plasmid
    #123182
    Purpose
    Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)
    Depositor
    Carolyn Lee-Parsons, Erin Cram
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
    Use
    Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites; codon-optimised for plants
    Promoter
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123182-plasmid-map-sequence-id-241413

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  11. pQCXIP GFP-MOSPD2 W201E

    Plasmid
    #186471
    Purpose
    Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to EGFP in mammalian cells
    Depositor
    Catherine Tomasetto
    Article
    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213
    Insert
    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    W201E (no more binding to lipid droplets)
    Promoter
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186471-plasmid-map-sequence-id-366065

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  24. 3294_pETcon-SARS2-RBD_Omicron-BA1

    Plasmid
    #184408
    Purpose
    yeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBD
    Depositor
    Jesse Bloom
    Article
    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1
    Insert
    SARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    Use
    Tags
    HA and c-Myc
    Expression
    Yeast
    Mutation
    G339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    184408-plasmid-map-sequence-id-362479

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  26. PKAsub(TA)-TagRFPT-CAAX

    Plasmid
    #87740
    Purpose
    Negative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    PKAsub(TA)-TagRFPT-CAAX
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87740-plasmid-map-sequence-id-176201

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  27. Tom20-mChF-AIP(TD)

    Plasmid
    #61521
    Purpose
    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TD mutation
    Depositor
    Takanari Inoue
    Article
    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:
    Insert
    FK506 binding protein 1A (FKBP1A Human)
    Use
    Tags
    AIP with TD mutation (see comments), Tom20, and m…
    Expression
    Mammalian
    Mutation
    TD mutation (see comments)
    Promoter
    CMV
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  28. pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

    Plasmid
    #225939
    Purpose
    Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Evan Reid, Bart Nieuwenhuis
    Inserts
    STIM1 (STIM1 Human)
    Emerald
    Use
    Lentiviral
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225939-plasmid-map-sequence-id-446835

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  29. pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald

    Plasmid
    #225944
    Purpose
    Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Evan Reid, Bart Nieuwenhuis
    Inserts
    ATL1 (ATL1 Human)
    Emerald
    Use
    Lentiviral
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225944-plasmid-map-sequence-id-446838

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  39. Anti-Synaptotagmin-12 [N277/26R]

    Plasmid
    #225386
    Purpose
    Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse) IgG2a R-mAb. Derived from hybridoma N277/26.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Synaptotagmin-12 (Mus musculus) recombinant (Mouse) monoclonal antibody. (Syt12 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    225386-plasmid-map-sequence-id-449387

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  42. Anti-Synaptotagmin-12 [N277/44R]

    Plasmid
    #225387
    Purpose
    Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse) IgG2a R-mAb. Derived from hybridoma N277/44.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Synaptotagmin-12 (Mus musculus) recombinant (Mouse) monoclonal antibody. (Syt12 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    225387-plasmid-map-sequence-id-449379

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Showing: 2901 - 2950 of 4641 results